Structure/function analysis of a critical disulfide bond in the active site of l-xylulose reductase

Hai-Tao Zhao, Satoshi Endo, Shuhei Ishikura, Roland Poh-Tuck Chung, Phillip J Hogg, Akira Hara, Ossama El-Kabbani

Research output: Contribution to journalArticleResearchpeer-review

4 Citations (Scopus)

Abstract

l-Xylulose reductase (XR) is involved in water re-absorption and cellular osmoregulation. The crystal structure of human XR complemented with site-directed mutagenesis (Cys138Ala) indicated that the disulfide bond in the active site between Cys138 and Cys150 is unstable and may affect the reactivity of the enzyme. The effects of reducing agents on the activities of the wild-type and mutant enzymes indicated the reversibility of disulfide-bond formation, which resulted in three-fold decrease in catalytic efficiency. Furthermore, the addition of cysteine (> 2 mM) inactivated human XR and was accompanied by a 10-fold decrease in catalytic efficiency. TOF-MS analysis of the inactivated enzyme showed the S-cysteinylation of Cys138 in the wild-type and Cys150 in the mutant enzymes. Thus, the action of human XR may be regulated by cellular redox conditions through reversible disulfide-bond formation and by S-cysteinylation.
Original languageEnglish
Pages (from-to)1570 - 1579
Number of pages10
JournalCellular and Molecular Life Sciences
Volume66
Issue number9
Publication statusPublished - 2009

Cite this