Structure of the RESA gene of Piasmodium falciparum

J. M. Favaloro, R. L. Coppel, L. M. Corcoran, S. J. Foote, G. V. Brown, R. F. Anders, D. J. Kemp

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Abstract

We have determined the nucleotide sequence of the gene encoding the ring-infected erythrocyte surface antigen (RESA) of Plasmodiua falciparum, an antigen that has been shown to confer protective immunity on monkeys. The sequence has enabled us to predict the structure of the RESA gene and the amino acid sequence of its protein product. The gene consists of two exons with a short intron located near the 5′ end of the coding region. A hydro-phobic amino acid segment predicted for the 3′ end of exon 1 is consistent with the possibility that exon 1 encodes trafficking signal sequences. We show that restriction fragment length polymorphisms can be used to define two different alleles of RESA, represented by isolates FC27 and NF7, and compare the FC27 sequence with that of a long cDNA clone from NF7 described previously.

Original languageEnglish
Pages (from-to)8265-8277
Number of pages13
JournalNucleic Acids Research
Volume14
Issue number21
DOIs
Publication statusPublished - 11 Nov 1986
Externally publishedYes

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