Structure of the mitochondrial import gate reveals distinct preprotein paths

Yuhei Araiso, Akihisa Tsutsumi, Jian Qiu, Kenichiro Imai, Takuya Shiota, Jiyao Song, Caroline Lindau, Lena Sophie Wenz, Haruka Sakaue, Kaori Yunoki, Shin Kawano, Junko Suzuki, Marilena Wischnewski, Conny Schütze, Hirotaka Ariyama, Toshio Ando, Thomas Becker, Trevor Lithgow, Nils Wiedemann, Nikolaus PfannerMasahide Kikkawa, Toshiya Endo

Research output: Contribution to journalArticleResearchpeer-review

33 Citations (Scopus)

Abstract

The translocase of the outer mitochondrial membrane (TOM) is the main entry gate for proteins1–4. Here we use cryo-electron microscopy to report the structure of the yeast TOM core complex5–9 at 3.8-Å resolution. The structure reveals the high-resolution architecture of the translocator consisting of two Tom40 β-barrel channels and α-helical transmembrane subunits, providing insight into critical features that are conserved in all eukaryotes1–3. Each Tom40 β-barrel is surrounded by small TOM subunits, and tethered by two Tom22 subunits and one phospholipid. The N-terminal extension of Tom40 forms a helix inside the channel; mutational analysis reveals its dual role in early and late steps in the biogenesis of intermembrane-space proteins in cooperation with Tom5. Each Tom40 channel possesses two precursor exit sites. Tom22, Tom40 and Tom7 guide presequence-containing preproteins to the exit in the middle of the dimer, whereas Tom5 and the Tom40 N extension guide preproteins lacking a presequence to the exit at the periphery of the dimer.

Original languageEnglish
Pages (from-to)395-401
Number of pages7
JournalNature
Volume575
Issue number7782
DOIs
Publication statusPublished - 14 Nov 2019

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