Structure of the Haemagglutinin-neuraminidase from Human Parainfluenza Virus Type III

Michael C. Lawrence, Natalie A. Borg, Victor A. Streltsov, Patricia A. Pilling, V. Chandana Epa, Joseph N. Varghese, Jennifer L. McKimm-Breschkin, Peter M. Colman

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Abstract

The three-dimensional structure of the haemagglutinin-neuraminidase (HN) from a human parainfluenza virus is described at ca 2.0Å resolution, both in native form and in complex with three substrate analogues. In support of earlier work on the structure of the homologous protein from the avian pathogen Newcastle disease virus (NDV), we observe a dimer of β-propellers and find no evidence for spatially separated sites performing the receptor-binding and neuraminidase functions of the protein. As with the NDV HN, the active site of the HN of parainfluenza viruses is structurally flexible, suggesting that it may be able to switch between a receptor-binding state and a catalytic state. However, in contrast to the NDV structures, we observe no ligand-induced structural changes that extend beyond the active site and modify the dimer interface. Crown

Original languageEnglish
Pages (from-to)1343-1357
Number of pages15
JournalJournal of Molecular Biology
Volume335
Issue number5
DOIs
Publication statusPublished - 30 Jan 2004

Keywords

  • Haemagglutinin-neuraminidase
  • Parainfluenza
  • Paramyxovirus
  • Protein structure
  • Sialic acid

Cite this

Lawrence, M. C., Borg, N. A., Streltsov, V. A., Pilling, P. A., Epa, V. C., Varghese, J. N., ... Colman, P. M. (2004). Structure of the Haemagglutinin-neuraminidase from Human Parainfluenza Virus Type III. Journal of Molecular Biology, 335(5), 1343-1357. https://doi.org/10.1016/j.jmb.2003.11.032
Lawrence, Michael C. ; Borg, Natalie A. ; Streltsov, Victor A. ; Pilling, Patricia A. ; Epa, V. Chandana ; Varghese, Joseph N. ; McKimm-Breschkin, Jennifer L. ; Colman, Peter M. / Structure of the Haemagglutinin-neuraminidase from Human Parainfluenza Virus Type III. In: Journal of Molecular Biology. 2004 ; Vol. 335, No. 5. pp. 1343-1357.
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Lawrence, MC, Borg, NA, Streltsov, VA, Pilling, PA, Epa, VC, Varghese, JN, McKimm-Breschkin, JL & Colman, PM 2004, 'Structure of the Haemagglutinin-neuraminidase from Human Parainfluenza Virus Type III', Journal of Molecular Biology, vol. 335, no. 5, pp. 1343-1357. https://doi.org/10.1016/j.jmb.2003.11.032

Structure of the Haemagglutinin-neuraminidase from Human Parainfluenza Virus Type III. / Lawrence, Michael C.; Borg, Natalie A.; Streltsov, Victor A.; Pilling, Patricia A.; Epa, V. Chandana; Varghese, Joseph N.; McKimm-Breschkin, Jennifer L.; Colman, Peter M.

In: Journal of Molecular Biology, Vol. 335, No. 5, 30.01.2004, p. 1343-1357.

Research output: Contribution to journalArticleResearchpeer-review

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