Structure of the activation domain of the GM-CSF/IL-3/IL-5 receptor common β-chain bound to an antagonist

Jamie Rossjohn, William J. McKinstry, Joanna M. Woodcock, Barbara J. McClure, Timothy R. Hercus, Michael W. Parker, Angel F. Lopez, Christopher J. Bagley

Research output: Contribution to journalArticleResearchpeer-review

44 Citations (Scopus)

Abstract

Heterodimeric cytokine receptors generally consist of a major cytokine- binding subunit and a signaling subunit. The latter can transduce signals by more than 1 cytokine, as exemplified by the granulocyte-macrophage colony- stimulating factor (GM-CSF), interleukin-2 (IL-2), and IL-6 receptor systems. However, often the signaling subunits in isolation are unable to bind cytokines, a fact that has made it more difficult to obtain structural definition of their ligand-binding sites. This report details the crystal structure of the ligand-binding domain of the GM-CSF/IL-3/IL-5 receptor β- chain (β(c)) signaling subunit in complex with the Fab fragment of the antagonistic monoclonal antibody, BION-1. This is the first single antagonist of all 3 known eosinophil-producing cytokines, and it is therefore capable of regulating eosinophil-related diseases such as asthma. The structure reveals a fibronectin type III domain, and the antagonist-binding site involves major contributions from the loop between the B and C strands and overlaps the cytokine-binding site. Furthermore, tyrosine421 (Tyr421), a key residue involved in receptor activation, lies in the neighboring loop between the F and G strands, although it is not immediately adjacent to the cytokine- binding residues in the B-C loop. Interestingly, functional experiments using receptors mutated across these loops demonstrate that they are cooperatively involved in full receptor activation. The experiments, however, reveal subtle differences between the B-C loop and Tyr421, which is suggestive of distinct functional roles. The elucidation of the structure of the ligand- binding domain of β(c) also suggests how different cytokines recognize a single receptor subunit, which may have implications for homologous receptor systems. (C) 2000 by The American Society of Hematology.

Original languageEnglish
Pages (from-to)2491-2498
Number of pages8
JournalBlood
Volume95
Issue number8
Publication statusPublished - 15 Apr 2000

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