Structure of human MDM2 complexed with RPL11 reveals the molecular basis of p53 activation

Jiangge Zheng, Yue Lang, Qi Zhang, Di Cui, Haili Sun, Lun Jiang, Zhenhang Chen, Rui Zhang, Yina Gao, Wenli Tian, Wei Wu, Jun Tang, Zhongzhou Chen

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36 Citations (Scopus)

Abstract

The central region of MDM2 is critical for p53 activation and tumor suppression. Upon ribosomal stress, this region is bound by ribosomal proteins, particularly ribosomal protein L11 (RPL11), leading to MDM2 inactivation and subsequent p53 activation. Here, we solved the complex structure of human MDM2-RPL11 at 2.4 Å. MDM2 extensively interacts with RPL11 through an acidic domain and two zinc fingers. Formation of the MDM2-RPL11 complex induces substantial conformational changes in both proteins. RPL11, unable to bind MDM2 mutants, fails to induce the activation of p53 in cells. MDM2 mimics 28S rRNA binding to RPL11. The C4 zinc finger determines RPL11 binding to MDM2 but not its homolog, MDMX. Our results highlight the essential role of the RPL11-MDM2 interaction in p53 activation and tumor suppression and provide a structural basis for potential new anti-tumor drug development.
Original languageEnglish
Pages (from-to)1524-1534
Number of pages11
JournalGenes & Development
Volume29
Issue number14
DOIs
Publication statusPublished - 2015
Externally publishedYes

Keywords

  • Cancer
  • Complex structure
  • Molecular basis
  • p53 activation
  • RPL11—MDM2

Cite this

Zheng, J., Lang, Y., Zhang, Q., Cui, D., Sun, H., Jiang, L., Chen, Z., Zhang, R., Gao, Y., Tian, W., Wu, W., Tang, J., & Chen, Z. (2015). Structure of human MDM2 complexed with RPL11 reveals the molecular basis of p53 activation. Genes & Development, 29(14), 1524-1534. https://doi.org/10.1101/gad.261792.115