Structure of Est3 reveals a bimodal surface with differential roles in telomere replication

Timsi Rao, Johnathan W. Lubin, Geoffrey S. Armstrong, Timothy M. Tucey, Victoria Lundblad, Deborah S. Wuttke

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27 Citations (Scopus)

Abstract

Telomerase is essential for continuous cellular proliferation. Substantial insights have come from studies of budding yeast telomerase, which consists of a catalytic core in association with two regulatory proteins, ever shorter telomeres 1 and 3 (Est1 and Est3). We report here a high-resolution structure of the Est3 telomerase subunit determined using a recently developed strategy that combines minimal NMR experimental data with Rosetta de novo structure prediction algorithms. Est3 adopts an overall protein fold which is structurally similar to that adopted by the shelterin component TPP1. However, the characteristics of the surface of the experimentally determined Est3 structure are substantially different from those predicted by prior homology-based models of Est3. Structure-guided mutagenesis of the complete surface of the Est3 protein reveals two adjacent patches on a noncanonical face of the protein that differentially mediate telomere function. Mapping these two patches on the Est3 structure defines a set of shared features between Est3 and HsTPP1, suggesting an analogous multifunctional surface on TPP1.

Original languageEnglish
Pages (from-to)214-218
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume111
Issue number1
DOIs
Publication statusPublished - 7 Jan 2014
Externally publishedYes

Keywords

  • OB-fold protein
  • RASREC Rosetta

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