Structural Basis of Host Cell Recognition by the Pilus Adhesin from Streptococcus pneumoniae

Thierry Izoré, Carlos Contreras-Martel, Lamya El Mortaji, Clothilde Manzano, Rémy Terrasse, Thierry Vernet, Anne Marie Di Guilmi, Andréa Dessen

Research output: Contribution to journalArticleResearchpeer-review

82 Citations (Scopus)

Abstract

Pili are fibrous virulence factors associated directly to the bacterial surface that play critical roles in adhesion and recognition of host cell receptors. The human pathogen Streptococcus pneumoniae carries a single pilus-related adhesin (RrgA) that is key for infection establishment and provides protection from bacterial challenge in animal infection models, but details of these roles remain unclear. Here we report the high-resolution crystal structure of RrgA, a 893-residue elongated macromolecule whose fold contains four domains presenting both eukaryotic and prokaryotic origins. RrgA harbors an integrin I collagen-recognition domain decorated with two inserted "arms" that fold into a positively charged cradle, as well as three "stalk-forming" domains. We show by site-specific mutagenesis, mass spectrometry, and thermal shift assays that intradomain isopeptide bonds play key roles in stabilizing RrgA's stalk. The high sequence similarity between RrgA and its homologs in other Gram-positive microorganisms suggests common strategies for ECM recognition and immune evasion.

Original languageEnglish
Pages (from-to)106-115
Number of pages10
JournalStructure
Volume18
Issue number1
DOIs
Publication statusPublished - 13 Jan 2010
Externally publishedYes

Keywords

  • MICROBIO
  • PROTEINS

Cite this

Izoré, T., Contreras-Martel, C., El Mortaji, L., Manzano, C., Terrasse, R., Vernet, T., ... Dessen, A. (2010). Structural Basis of Host Cell Recognition by the Pilus Adhesin from Streptococcus pneumoniae. Structure, 18(1), 106-115. https://doi.org/10.1016/j.str.2009.10.019
Izoré, Thierry ; Contreras-Martel, Carlos ; El Mortaji, Lamya ; Manzano, Clothilde ; Terrasse, Rémy ; Vernet, Thierry ; Di Guilmi, Anne Marie ; Dessen, Andréa. / Structural Basis of Host Cell Recognition by the Pilus Adhesin from Streptococcus pneumoniae. In: Structure. 2010 ; Vol. 18, No. 1. pp. 106-115.
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abstract = "Pili are fibrous virulence factors associated directly to the bacterial surface that play critical roles in adhesion and recognition of host cell receptors. The human pathogen Streptococcus pneumoniae carries a single pilus-related adhesin (RrgA) that is key for infection establishment and provides protection from bacterial challenge in animal infection models, but details of these roles remain unclear. Here we report the high-resolution crystal structure of RrgA, a 893-residue elongated macromolecule whose fold contains four domains presenting both eukaryotic and prokaryotic origins. RrgA harbors an integrin I collagen-recognition domain decorated with two inserted {"}arms{"} that fold into a positively charged cradle, as well as three {"}stalk-forming{"} domains. We show by site-specific mutagenesis, mass spectrometry, and thermal shift assays that intradomain isopeptide bonds play key roles in stabilizing RrgA's stalk. The high sequence similarity between RrgA and its homologs in other Gram-positive microorganisms suggests common strategies for ECM recognition and immune evasion.",
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Izoré, T, Contreras-Martel, C, El Mortaji, L, Manzano, C, Terrasse, R, Vernet, T, Di Guilmi, AM & Dessen, A 2010, 'Structural Basis of Host Cell Recognition by the Pilus Adhesin from Streptococcus pneumoniae', Structure, vol. 18, no. 1, pp. 106-115. https://doi.org/10.1016/j.str.2009.10.019

Structural Basis of Host Cell Recognition by the Pilus Adhesin from Streptococcus pneumoniae. / Izoré, Thierry; Contreras-Martel, Carlos; El Mortaji, Lamya; Manzano, Clothilde; Terrasse, Rémy; Vernet, Thierry; Di Guilmi, Anne Marie; Dessen, Andréa.

In: Structure, Vol. 18, No. 1, 13.01.2010, p. 106-115.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Izoré, Thierry

AU - Contreras-Martel, Carlos

AU - El Mortaji, Lamya

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AU - Terrasse, Rémy

AU - Vernet, Thierry

AU - Di Guilmi, Anne Marie

AU - Dessen, Andréa

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Izoré T, Contreras-Martel C, El Mortaji L, Manzano C, Terrasse R, Vernet T et al. Structural Basis of Host Cell Recognition by the Pilus Adhesin from Streptococcus pneumoniae. Structure. 2010 Jan 13;18(1):106-115. https://doi.org/10.1016/j.str.2009.10.019