Structural basis for chain release from the enacyloxin polyketide synthase

Simone Kosol, Angelo Gallo, Daniel Griffiths, Timothy R. Valentic, Joleen Masschelein, Matthew Jenner, Emmanuel L.C. de Los Santos, Lucio Manzi, Paulina K. Sydor, Dean Rea, Shanshan Zhou, Vilmos Fülöp, Neil J. Oldham, Shiou Chuan Tsai, Gregory L. Challis, Józef R. Lewandowski

Research output: Contribution to journalArticleResearchpeer-review

16 Citations (Scopus)

Abstract

Modular polyketide synthases and non-ribosomal peptide synthetases are molecular assembly lines that consist of several multienzyme subunits that undergo dynamic self-assembly to form a functional megacomplex. N- and C-terminal docking domains are usually responsible for mediating the interactions between subunits. Here we show that communication between two non-ribosomal peptide synthetase subunits responsible for chain release from the enacyloxin polyketide synthase, which assembles an antibiotic with promising activity against Acinetobacter baumannii, is mediated by an intrinsically disordered short linear motif and a β-hairpin docking domain. The structures, interactions and dynamics of these subunits were characterized using several complementary biophysical techniques to provide extensive insights into binding and catalysis. Bioinformatics analyses reveal that short linear motif/β-hairpin docking domain pairs mediate subunit interactions in numerous non-ribosomal peptide and hybrid polyketide-non-ribosomal peptide synthetases, including those responsible for assembling several important drugs. Short linear motifs and β-hairpin docking domains from heterologous systems are shown to interact productively, highlighting the potential of such interfaces as tools for biosynthetic engineering.

Original languageEnglish
Pages (from-to)913-923
Number of pages11
JournalNature Chemistry
Volume11
Issue number10
DOIs
Publication statusPublished - 1 Oct 2019

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