Stress-induced changes in plasma concentrations of immunoreactive β-endorphin and cortisol in response to routine surgical procedures in lambs

D. A. Shutt, L. R. Fell, R. Connell, A. K. Bell, C. A. Wallace, A. I. Smith

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Abstract

Following four different surgical procedures in lambs 3–5 weeks old, plasma immunoreactive β-endorphin (β-EP) and cortisol were assayed at 15 min and 24 h as determinants of post-operative stress. A threefold increase in mean plasma β-EP levels occurred 15 min after tail docking, and a maximal eight-to tenfold increase occurred in response to castration and/or mulesing with tail docking. Significant increments in mean plasma cortisol levels followed these surgical procedures with the maximal response 15 min after mulesing plus castration with tail docking. The physiologically active ‘free’ cortisol in plasma represents about 25% of the cortisol, as measured, and the two are highly correlated. At 24 h, β-EP levels in all. treated groups were similar to controls, although a small elevation in cortisol levels was still present in the lambs subjected to mulesing. Ultrafiltration of plasma extracts showed that peak β-EP levels contained about 40% immunoreactivity from low molecular weight species (mol. wt < 10 000). By specific radioimmunoassay and reverse-phase high-performance liquid chromatography this comprised about 75% β-EP1−31, the most potent analgesic endorphin, 10% β-EP1−27, and 15% α-N-acetyl-β-EP. Increased β-EP1−31 levels may modulate post-operative pain in lambs.

Original languageEnglish
Pages (from-to)97-104
Number of pages8
JournalAustralian Journal of Biological Sciences
Volume40
Issue number1
DOIs
Publication statusPublished - 1 Jan 1987
Externally publishedYes

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