An effective strategy for the quantitative and sensitive colorimetric detection of H2O2 and glucose by combining a novel concept of stimuli-triggered charge-generation polymer (termed as CGP) with gold nanoparticles (AuNPs) was investigated. A new polymerizable monomer, PBMA, was designed and synthesized, through the reaction of boronate ester-functionalized benzyl alcohol derivative with 2-isocyanatoethyl methacrylate. As PBMA monomer lacks sufficient water solubility, it was opted to synthesize PBMA-containing water-soluble polymers through the free radical copolymerization of PBMA with N, N-dimethylacrylamide (DMA). The obtained P(DMA-co-PBMA) copolymer was subjected to further fractionation to afford relatively narrow-disperse samples. 1H NMR and gel permeation chromatography analysis revealed an overall degree of polymerization (DP) of 620, an Mw/Mn of 1.27, and a PBMA molar content of 11 .