TY - JOUR
T1 - Stiffness-Dependent Intracellular Location of Cylindrical Polymer Brushes
AU - Niederberger, Antoine
AU - Pelras, Théophile
AU - Manni, Livia Salvati
AU - FitzGerald, Paul A.
AU - Warr, Gregory G.
AU - Müllner, Markus
N1 - Funding Information:
The authors greatly acknowledge the free use of SasView (http://www.sasview.org/), developed under the NSF award DMR-0520547, SINE2020 project, grant agreement No 654000. The authors thank Prof. Chiara Neto for providing access to atomic force microscopes and the Key Centre for Polymers and Colloids (KCPC) for access to equipment. T.P. was a grateful recipient of a Sydney Nano Postgraduate Top-Up Scholarship. L.S.M., G.G.W. and M.M. acknowledge support by the Schofield Fund. T.P., P.F., G.G.W., and M.M. thank the Australian Nuclear Science and Technology Organization (ANSTO, P5694) for beam line access on Quokka. M.M. acknowledges the Australian Research Council (DE180100007, FT200100185) for support. This project was funded through an ARC DECRA (DE180100007).
Funding Information:
The authors greatly acknowledge the free use of SasView ( http://www.sasview.org/ ), developed under the NSF award DMR‐0520547, SINE2020 project, grant agreement No 654000. The authors thank Prof. Chiara Neto for providing access to atomic force microscopes and the Key Centre for Polymers and Colloids (KCPC) for access to equipment. T.P. was a grateful recipient of a Sydney Nano Postgraduate Top‐Up Scholarship. L.S.M., G.G.W. and M.M. acknowledge support by the Schofield Fund. T.P., P.F., G.G.W., and M.M. thank the Australian Nuclear Science and Technology Organization (ANSTO, P5694) for beam line access on Quokka. M.M. acknowledges the Australian Research Council (DE180100007, FT200100185) for support. This project was funded through an ARC DECRA (DE180100007).
Publisher Copyright:
© 2021 Wiley-VCH GmbH
PY - 2021/7
Y1 - 2021/7
N2 - Cylindrical polymer brushes (CPBs) are macromolecules with nanoparticle proportions. Their modular synthesis enables tailoring of their chemical composition as well as the dialing-up of overall dimensions and physicochemical properties. In this study, two rod-like poly[(ethylene glycol) methyl ether methacrylate] (PEGMA)-based CPBs with varying stiffness but otherwise comparable features and functionality, are synthesized. Differences in particle stiffness are assessed using small angle neutron scattering (SANS). It is observed that the fate of the two CPBs within cells is distinctly different. Stiffer CPBs seem to gravitate toward the mitochondria, whereas CPBs with reduced stiffness are present in different intracellular vesicles.
AB - Cylindrical polymer brushes (CPBs) are macromolecules with nanoparticle proportions. Their modular synthesis enables tailoring of their chemical composition as well as the dialing-up of overall dimensions and physicochemical properties. In this study, two rod-like poly[(ethylene glycol) methyl ether methacrylate] (PEGMA)-based CPBs with varying stiffness but otherwise comparable features and functionality, are synthesized. Differences in particle stiffness are assessed using small angle neutron scattering (SANS). It is observed that the fate of the two CPBs within cells is distinctly different. Stiffer CPBs seem to gravitate toward the mitochondria, whereas CPBs with reduced stiffness are present in different intracellular vesicles.
KW - cellular interactions
KW - molecular polymer brushes
KW - polymer bottlebrushes
KW - polymer nanoparticles
KW - stiffness
UR - http://www.scopus.com/inward/record.url?scp=85104420673&partnerID=8YFLogxK
U2 - 10.1002/marc.202100138
DO - 10.1002/marc.202100138
M3 - Article
C2 - 33871109
AN - SCOPUS:85104420673
SN - 1022-1336
VL - 42
JO - Macromolecular Rapid Communications
JF - Macromolecular Rapid Communications
IS - 13
M1 - 2100138
ER -