Stealth nanorods via the aqueous living crystallisation-driven self-assembly of poly(2-oxazoline)s

John R. Finnegan; Emily H. Pilkington; Karen Alt; Md Arifur Rahim; Stephen J. Kent; Thomas P. Davis; Kristian Kempe

doi:10.1039/d1sc00938a

Stealth nanorods via the aqueous living crystallisation-driven self-assembly of poly(2-oxazoline)s

John R. Finnegan, Emily H. Pilkington, Karen Alt, Md Arifur Rahim, Stephen J. Kent, Thomas P. Davis, Kristian Kempe

Research output: Contribution to journal › Article › Research › peer-review

38 Citations (Scopus)

Abstract

The morphology of nanomaterials critically influences their biological interactions. However, there is currently a lack of robust methods for preparing non-spherical particles from biocompatible materials. Here, we combine ‘living’ crystallisation-driven self-assembly (CDSA), a seeded growth method that enables the preparation of rod-like polymer nanoparticles, with poly(2-oxazoline)s (POx), a polymer class that exhibits ‘stealth’ behaviour and excellent biocompatibility. For the first time, the ‘living’ CDSA process was carried out in pure water, resulting in POx nanorods with lengths ranging from ∼60 to 635 nm.In vitroandin vivostudy revealed low immune cell association and encouraging blood circulation times, but little difference in the behaviour of POx nanorods of different length. The stealth behaviour observed highlights the promising potential of POx nanorods as a next generation stealth drug delivery platform.

Original language	English
Pages (from-to)	7350-7360
Number of pages	11
Journal	Chemical Science
Volume	12
Issue number	21
DOIs	https://doi.org/10.1039/d1sc00938a
Publication status	Published - 7 Jun 2021

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10.1039/d1sc00938aLicence: CC BY

Cite this

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title = "Stealth nanorods via the aqueous living crystallisation-driven self-assembly of poly(2-oxazoline)s",

abstract = "The morphology of nanomaterials critically influences their biological interactions. However, there is currently a lack of robust methods for preparing non-spherical particles from biocompatible materials. Here, we combine {\textquoteleft}living{\textquoteright} crystallisation-driven self-assembly (CDSA), a seeded growth method that enables the preparation of rod-like polymer nanoparticles, with poly(2-oxazoline)s (POx), a polymer class that exhibits {\textquoteleft}stealth{\textquoteright} behaviour and excellent biocompatibility. For the first time, the {\textquoteleft}living{\textquoteright} CDSA process was carried out in pure water, resulting in POx nanorods with lengths ranging from ∼60 to 635 nm.In vitroandin vivostudy revealed low immune cell association and encouraging blood circulation times, but little difference in the behaviour of POx nanorods of different length. The stealth behaviour observed highlights the promising potential of POx nanorods as a next generation stealth drug delivery platform.",

author = "Finnegan, {John R.} and Pilkington, {Emily H.} and Karen Alt and Rahim, {Md Arifur} and Kent, {Stephen J.} and Davis, {Thomas P.} and Kristian Kempe",

note = "Funding Information: K. K. gratefully acknowledges the award of an ARC Future Fellowship (FT190100572) from the Australian Research Council (ARC). K. A. is supported by the National Health and Medical Research Council of Australia (Career Development Fellowship GNT1140465). This work was – in part – carried out within the ARC Centre of Excellence in Convergent Bio-Nano Science and Technology (Project No. CE140100036). We would like to thank the Bio21 Advanced Microscopy Facility (University of Melbourne) and Prof. Patrick Sexton (Monash University) for access to their electron microscopy facilities. Finally, the authors acknowledge the use of the facilities and the assistance of Dr Jisheng Ma at the Monash X-ray Platform, as well as the support provided to this project by the HMS Trust Laboratory, based at the Monash Institute of Pharmaceutical Sciences. Funding Information: K. K. gratefully acknowledges the award of an ARC Future Fellowship (FT190100572) from the Australian Research Council (ARC). K. A. is supported by the National Health and Medical Research Council of Australia (Career Development Fellowship GNT1140465). This work was - in part - carried out within the ARC Centre of Excellence in Convergent Bio-Nano Science and Technology (Project No. CE140100036). We would like to thank the Bio21 Advanced Microscopy Facility (University of Melbourne) and Prof. Patrick Sexton (Monash University) for access to their electron microscopy facilities. Finally, the authors acknowledge the use of the facilities and the assistance of Dr Jisheng Ma at the Monash X-ray Platform, as well as the support provided to this project by the HMS Trust Laboratory, based at the Monash Institute of Pharmaceutical Sciences. Publisher Copyright: {\textcopyright} The Royal Society of Chemistry 2021. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.",

year = "2021",

month = jun,

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doi = "10.1039/d1sc00938a",

language = "English",

volume = "12",

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journal = "Chemical Science",

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AU - Rahim, Md Arifur

AU - Kent, Stephen J.

AU - Davis, Thomas P.

AU - Kempe, Kristian

N1 - Funding Information: K. K. gratefully acknowledges the award of an ARC Future Fellowship (FT190100572) from the Australian Research Council (ARC). K. A. is supported by the National Health and Medical Research Council of Australia (Career Development Fellowship GNT1140465). This work was – in part – carried out within the ARC Centre of Excellence in Convergent Bio-Nano Science and Technology (Project No. CE140100036). We would like to thank the Bio21 Advanced Microscopy Facility (University of Melbourne) and Prof. Patrick Sexton (Monash University) for access to their electron microscopy facilities. Finally, the authors acknowledge the use of the facilities and the assistance of Dr Jisheng Ma at the Monash X-ray Platform, as well as the support provided to this project by the HMS Trust Laboratory, based at the Monash Institute of Pharmaceutical Sciences. Funding Information: K. K. gratefully acknowledges the award of an ARC Future Fellowship (FT190100572) from the Australian Research Council (ARC). K. A. is supported by the National Health and Medical Research Council of Australia (Career Development Fellowship GNT1140465). This work was - in part - carried out within the ARC Centre of Excellence in Convergent Bio-Nano Science and Technology (Project No. CE140100036). We would like to thank the Bio21 Advanced Microscopy Facility (University of Melbourne) and Prof. Patrick Sexton (Monash University) for access to their electron microscopy facilities. Finally, the authors acknowledge the use of the facilities and the assistance of Dr Jisheng Ma at the Monash X-ray Platform, as well as the support provided to this project by the HMS Trust Laboratory, based at the Monash Institute of Pharmaceutical Sciences. Publisher Copyright: © The Royal Society of Chemistry 2021. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

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N2 - The morphology of nanomaterials critically influences their biological interactions. However, there is currently a lack of robust methods for preparing non-spherical particles from biocompatible materials. Here, we combine ‘living’ crystallisation-driven self-assembly (CDSA), a seeded growth method that enables the preparation of rod-like polymer nanoparticles, with poly(2-oxazoline)s (POx), a polymer class that exhibits ‘stealth’ behaviour and excellent biocompatibility. For the first time, the ‘living’ CDSA process was carried out in pure water, resulting in POx nanorods with lengths ranging from ∼60 to 635 nm.In vitroandin vivostudy revealed low immune cell association and encouraging blood circulation times, but little difference in the behaviour of POx nanorods of different length. The stealth behaviour observed highlights the promising potential of POx nanorods as a next generation stealth drug delivery platform.

AB - The morphology of nanomaterials critically influences their biological interactions. However, there is currently a lack of robust methods for preparing non-spherical particles from biocompatible materials. Here, we combine ‘living’ crystallisation-driven self-assembly (CDSA), a seeded growth method that enables the preparation of rod-like polymer nanoparticles, with poly(2-oxazoline)s (POx), a polymer class that exhibits ‘stealth’ behaviour and excellent biocompatibility. For the first time, the ‘living’ CDSA process was carried out in pure water, resulting in POx nanorods with lengths ranging from ∼60 to 635 nm.In vitroandin vivostudy revealed low immune cell association and encouraging blood circulation times, but little difference in the behaviour of POx nanorods of different length. The stealth behaviour observed highlights the promising potential of POx nanorods as a next generation stealth drug delivery platform.

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DO - 10.1039/d1sc00938a

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JO - Chemical Science

JF - Chemical Science

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ER -

Stealth nanorods via the aqueous living crystallisation-driven self-assembly of poly(2-oxazoline)s

Abstract

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Expanding the toolbox of synthetic stealth polymers

Novel targeted Onco-theranostic nanoparticles for personalised therapy and real-time monitoring

ARC Centre of Excellence in Convergent Bio-Nano Science and Technology

X-ray Platform (MXP)

Cite this

Stealth nanorods via the aqueous living crystallisation-driven self-assembly of poly(2-oxazoline)s

Abstract

Access to Document

Other files and links

Projects

Expanding the toolbox of synthetic stealth polymers

Novel targeted Onco-theranostic nanoparticles for personalised therapy and real-time monitoring

ARC Centre of Excellence in Convergent Bio-Nano Science and Technology

Equipment

X-ray Platform (MXP)

Cite this