TY - JOUR
T1 - Staufen2 regulates neuronal target RNAs
AU - Heraud-Farlow, Jacki E.
AU - Sharangdhar, Tejaswini
AU - Li, Xiao
AU - Pfeifer, Philipp
AU - Tauber, Stefanie
AU - Orozco, Denise
AU - Hörmann, Alexandra
AU - Thomas, Sabine
AU - Bakosova, Anetta
AU - Farlow, Ashley R.
AU - Edbauer, Dieter
AU - Lipshitz, Howard D.
AU - Morris, Quaid D.
AU - Bilban, Martin
AU - Doyle, Michael
AU - Kiebler, Michael A.
N1 - Funding Information:
We thank Christin Illig and Marco Tolino for assistance with preparation of primary neuron cultures, Daniela Karra for initial work involving RNA isolation for microarrays, and Dorothee Dormann, Marija Vukajlovic, and Jernej Ule for comments on the manuscript. This work was supported by the Austrian Science Fund (P20583-B12, I 590-B09, SFB F43), the ESF Program RNAQuality (I 127-B12), the Schram Foundation and an HFSP network grant (RGP24/2008) (all to M.A.K.), and Canadian Institutes of Health Research operating grants (MOP-125894 to Q.D.M. and MOP-14409 to H.D.L.). J.H. was supported by a PhD fellowship from the FWF (DK RNA Biology: W1207-B09). D.E. was supported by the Helmholtz Young Investigator program (HZ-NG-607).
PY - 2013/12/26
Y1 - 2013/12/26
N2 - RNA-binding proteins play crucial roles in directing RNA translation to neuronal synapses. Staufen2 (Stau2) has been implicated in both dendritic RNA localization and synaptic plasticity in mammalian neurons. Here, we report the identification of functionally relevant Stau2 target mRNAs in neurons. The majority of Stau2-copurifying mRNAs expressed in the hippocampus are present in neuronal processes, further implicating Stau2 in dendritic mRNA regulation. Stau2 targets are enriched for secondary structures similar to those identified in the 3' UTRs of Drosophila Staufen targets. Next, we show that Stau2 regulates steady-state levels of many neuronal RNAs and that its targets are predominantly downregulated in Stau2-deficient neurons. Detailed analysis confirms that Stau2 stabilizes the expression of one synaptic signaling component, the regulator of G protein signaling 4 (Rgs4) mRNA, via its 3' UTR. This study defines the global impact of Stau2 on mRNAs in neurons, revealing a role in stabilization of the levels of synaptic targets.
AB - RNA-binding proteins play crucial roles in directing RNA translation to neuronal synapses. Staufen2 (Stau2) has been implicated in both dendritic RNA localization and synaptic plasticity in mammalian neurons. Here, we report the identification of functionally relevant Stau2 target mRNAs in neurons. The majority of Stau2-copurifying mRNAs expressed in the hippocampus are present in neuronal processes, further implicating Stau2 in dendritic mRNA regulation. Stau2 targets are enriched for secondary structures similar to those identified in the 3' UTRs of Drosophila Staufen targets. Next, we show that Stau2 regulates steady-state levels of many neuronal RNAs and that its targets are predominantly downregulated in Stau2-deficient neurons. Detailed analysis confirms that Stau2 stabilizes the expression of one synaptic signaling component, the regulator of G protein signaling 4 (Rgs4) mRNA, via its 3' UTR. This study defines the global impact of Stau2 on mRNAs in neurons, revealing a role in stabilization of the levels of synaptic targets.
UR - http://www.scopus.com/inward/record.url?scp=84890990393&partnerID=8YFLogxK
U2 - 10.1016/j.celrep.2013.11.039
DO - 10.1016/j.celrep.2013.11.039
M3 - Article
C2 - 24360961
AN - SCOPUS:84890990393
SN - 2211-1247
VL - 5
SP - 1511
EP - 1518
JO - Cell Reports
JF - Cell Reports
IS - 6
ER -