Stanniocalcin-1, an inhibitor of macrophage chemotaxis and chemokinesis

John Kanellis, Roger Bick, Gabriela Garcia, Luan D Truong, Chun Chui Tsao, Dariush Etemadmoghadam, Brian Poindexter, Lili Feng, Richard J Johnson, David Sheikh-Hamad

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Abstract

In macrophages, changes in intracellular calcium have been associated with activation of cellular processes that regulate cell adhesion and motility and are important for the response of macrophages to antigenic stimuli. The mammalian counterpart of the fish calcium-regulating hormone stanniocalcin-1 (STC1) is expressed in multiple organs including the thymus and spleen, and hence, we hypothesized that it may have a role in modulating the immune/inflammatory response. Using murine macrophage-like (RAW264.7) and human monoblast-like (U937) cells to study chemotaxis in vitro, we found that STC1 attenuated chemokinesis and diminished the chemotactic response to monocyte chemotactic protein-1 (MCP-1) and stromal cell-derived factor-1alpha. Consistent with these findings, STC1 blunted the rise in intracellular calcium following MCP-1 stimulation in RAW264.7 cells. In vivo studies suggested differential expression of STC1 in obstructed kidney and localization to macrophages. MCP-1 and STC1 transcripts were both upregulated following ureteric obstruction, suggesting a functional association between the two genes. Our data suggest a role for mammalian STC1 in modulating the immune/inflammatory response.
Original languageEnglish
Pages (from-to)356 - 362
Number of pages7
JournalAmerican Journal of Physiology-Renal Physiology
Volume286
Issue number2
DOIs
Publication statusPublished - 2004
Externally publishedYes

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