@inbook{293bcab482524099bb57c1ad5717937b,
title = "Staining MIF in Cells for Confocal Microscopy",
abstract = "Confocal microscopy is a powerful technique for immunofluorescence imaging of cells and tissues. The technique allows for detailed analysis of intracellular localization of molecules, as well as three-dimensional representation and analysis of samples, and can be used as a gateway to more advanced techniques, including FLIM-FRET and super-resolution microscopy. Relatively few studies have used confocal microscopy to study intracellular localization of macrophage migration inhibitory factor (MIF) in detail. This chapter outlines basic protocols and tips for staining MIF in fixed cells for confocal analysis.",
keywords = "Antibody staining, Confocal, Fluorophores, Immunofluorescence",
author = "James Harris",
year = "2020",
month = jan,
day = "1",
doi = "10.1007/978-1-4939-9936-1_8",
language = "English",
isbn = "9781493999354",
series = "Methods in Molecular Biology: Springer Protocols",
publisher = "Humana Press",
pages = "85--91",
editor = "Harris, {James } and Morand, {Eric }",
booktitle = "Macrophage Migration Inhibitory Factor",
address = "United States of America",
}