Stable transgene expression inhumanembryonic stem cells after simple chemical transfection

Jun Liu; Karen Louise Jones; Huseyin Sumer; Paul John Verma

doi:10.1002/mrd.20983

Stable transgene expression inhumanembryonic stem cells after simple chemical transfection

Jun Liu
, Karen Louise Jones
, Huseyin Sumer
, Paul John Verma

Research output: Contribution to journal › Article › Research › peer-review

33 Citations (Scopus)

Abstract

In this study we used plasmid-based vectors to investigate the transcriptional activities of three commonly used promoters in transient and stable transfection of MEL-1, a human embryonic stem (ES) cell line, using ExGen500, Fugene HD, and Lipofectamine

Original language	English
Pages (from-to)	580 - 586
Number of pages	6
Journal	Molecular Reproduction and Development
Volume	76
DOIs	https://doi.org/10.1002/mrd.20983
Publication status	Published - 2009

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10.1002/mrd.20983

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title = "Stable transgene expression inhumanembryonic stem cells after simple chemical transfection",

abstract = "In this study we used plasmid-based vectors to investigate the transcriptional activities of three commonly used promoters in transient and stable transfection of MEL-1, a human embryonic stem (ES) cell line, using ExGen500, Fugene HD, and Lipofectamine",

author = "Jun Liu and Jones, \{Karen Louise\} and Huseyin Sumer and Verma, \{Paul John\}",

year = "2009",

doi = "10.1002/mrd.20983",

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journal = "Molecular Reproduction and Development",

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AU - Liu, Jun

AU - Jones, Karen Louise

AU - Sumer, Huseyin

AU - Verma, Paul John

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Y1 - 2009

N2 - In this study we used plasmid-based vectors to investigate the transcriptional activities of three commonly used promoters in transient and stable transfection of MEL-1, a human embryonic stem (ES) cell line, using ExGen500, Fugene HD, and Lipofectamine

AB - In this study we used plasmid-based vectors to investigate the transcriptional activities of three commonly used promoters in transient and stable transfection of MEL-1, a human embryonic stem (ES) cell line, using ExGen500, Fugene HD, and Lipofectamine

UR - http://www3.interscience.wiley.com/journal/122313200/issue

U2 - 10.1002/mrd.20983

DO - 10.1002/mrd.20983

M3 - Article

SN - 1040-452X

VL - 76

SP - 580

EP - 586

JO - Molecular Reproduction and Development

JF - Molecular Reproduction and Development

ER -

Stable transgene expression inhumanembryonic stem cells after simple chemical transfection

Abstract

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