Abstract
Placental growth factor (PlGF), total soluble fms-like tyrosine-kinase 1 (sFlt-1) and its placental-specific variant, sFlt-1 e15a, show promise as biomarkers for the prediction and diagnosis of preeclampsia. This study describes the degradation of PlGF, sFlt-1 and sFlt-1 e15a within maternal serum and plasma to assist clinical implementation. Whole blood was refrigerated at 4 °C for up to 48 h prior to centrifugation for isolation of plasma and serum. PlGF and sFlt-1 were quantified using the B.R.A.H.M.S Kryptor Compact PLUS; sFlt-1 e15a via a custom ELISA. All three analytes are stable for at least 48 h at 4 °C. Serum and plasma performed comparably.
Original language | English |
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Pages (from-to) | 1-3 |
Number of pages | 3 |
Journal | Placenta |
Volume | 86 |
DOIs | |
Publication status | Published - 1 Oct 2019 |
Keywords
- Anti-angiogenic factors
- PlGF
- Preeclampsia
- sFlt-1
- sFlt-1 e15a