Stability of gastrointestinal glutathione peroxidase mRNA in selenium deficiency depends on its 3'UTR

Kirstin Wingler, Cordula Muller, Regina Brigelius-Flohe

Research output: Contribution to journalArticleResearchpeer-review

12 Citations (Scopus)

Abstract

Selenoproteins decrease upon selenium-deprivation according to their hierarchical ranking. Whereas classical glutathione peroxidase (cGPx) responds to decreased selenium supply with a complete loss of protein and a marked reduction of mRNA levels, gastrointestinal glutathione peroxidase (GI-GPx) remains detectable and its mRNA is stable. The impact of the 3 UTR on cGPx and GI-GPx mRNA stability was studied in stably transfected HepG2 cells with combinations of mutually exchanged coding regions and 3 UTRs of human cGPx and GI-GPx. Stability of chimeric mRNAs was measured by competitive RT-PCR. We found that GI-GPx 3 UTR is sufficient to stabilize its own mRNA but not that of cGPx.
Original languageEnglish
Pages (from-to)43 - 50
Number of pages8
JournalBioFactors
Volume14
Issue number1-4
Publication statusPublished - 2001

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