SseK3 is a Salmonella effector that binds TRIM32 and modulates the host's NF-κB signalling activity

Zhe Yang, Amelia Soderholm, Tania Wong Fok Lung, Cristina Giogha, Michelle Hill, Nathaniel F. Brown, Elizabeth Hartland, Rohan D. Teasdale

Research output: Contribution to journalArticleResearchpeer-review

19 Citations (Scopus)

Abstract

Salmonella Typhimurium employs an array of type III secretion system effectors that facilitate intracellular survival and replication during infection. The Salmonella effector SseK3 was originally identified due to amino acid sequence similarity with NleB; an effector secreted by EPEC/EHEC that possesses N-acetylglucoasmine (GlcNAc) transferase activity and modifies death domain containing proteins to block extrinsic apoptosis. In this study, immunoprecipitation of SseK3 defined a novel molecular interaction between SseK3 and the host protein, TRIM32, an E3 ubiquitin ligase. The conserved DxD motif within SseK3, which is essential for the GlcNAc transferase activity of NleB, was required for TRIM32 binding and for the capacity of SseK3 to suppress TNF-stimulated activation of NF-κB pathway. However, we did not detect GlcNAc modification of TRIM32 by SseK3, nor did the SseK3-TRIM32 interaction impact on TRIM32 ubiquitination that is associated with its activation. In addition, lack of sseK3 in Salmonella had no effect on production of the NF-κB dependent cytokine, IL-8, in HeLa cells even though TRIM32 knockdown suppressed TNF-induced NF-κB activity. Ectopically expressed SseK3 partially co-localises with TRIM32 at the trans-Golgi network, but SseK3 is not recruited to Salmonella induced vacuoles or Salmonella induced filaments during Salmonella infection. Our study has identified a novel effector-host protein interaction and suggests that SseK3 may influence NF-κB activity. However, the lack of GlcNAc modification of TRIM32 suggests that SseK3 has further, as yet unidentified, host targets.

Original languageEnglish
Article numbere0138529
Number of pages20
JournalPLoS ONE
Volume10
Issue number9
DOIs
Publication statusPublished - 22 Sep 2015
Externally publishedYes

Cite this

Yang, Z., Soderholm, A., Lung, T. W. F., Giogha, C., Hill, M., Brown, N. F., ... Teasdale, R. D. (2015). SseK3 is a Salmonella effector that binds TRIM32 and modulates the host's NF-κB signalling activity. PLoS ONE, 10(9), [e0138529]. https://doi.org/10.1371/journal.pone.0138529
Yang, Zhe ; Soderholm, Amelia ; Lung, Tania Wong Fok ; Giogha, Cristina ; Hill, Michelle ; Brown, Nathaniel F. ; Hartland, Elizabeth ; Teasdale, Rohan D. / SseK3 is a Salmonella effector that binds TRIM32 and modulates the host's NF-κB signalling activity. In: PLoS ONE. 2015 ; Vol. 10, No. 9.
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abstract = "Salmonella Typhimurium employs an array of type III secretion system effectors that facilitate intracellular survival and replication during infection. The Salmonella effector SseK3 was originally identified due to amino acid sequence similarity with NleB; an effector secreted by EPEC/EHEC that possesses N-acetylglucoasmine (GlcNAc) transferase activity and modifies death domain containing proteins to block extrinsic apoptosis. In this study, immunoprecipitation of SseK3 defined a novel molecular interaction between SseK3 and the host protein, TRIM32, an E3 ubiquitin ligase. The conserved DxD motif within SseK3, which is essential for the GlcNAc transferase activity of NleB, was required for TRIM32 binding and for the capacity of SseK3 to suppress TNF-stimulated activation of NF-κB pathway. However, we did not detect GlcNAc modification of TRIM32 by SseK3, nor did the SseK3-TRIM32 interaction impact on TRIM32 ubiquitination that is associated with its activation. In addition, lack of sseK3 in Salmonella had no effect on production of the NF-κB dependent cytokine, IL-8, in HeLa cells even though TRIM32 knockdown suppressed TNF-induced NF-κB activity. Ectopically expressed SseK3 partially co-localises with TRIM32 at the trans-Golgi network, but SseK3 is not recruited to Salmonella induced vacuoles or Salmonella induced filaments during Salmonella infection. Our study has identified a novel effector-host protein interaction and suggests that SseK3 may influence NF-κB activity. However, the lack of GlcNAc modification of TRIM32 suggests that SseK3 has further, as yet unidentified, host targets.",
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Yang, Z, Soderholm, A, Lung, TWF, Giogha, C, Hill, M, Brown, NF, Hartland, E & Teasdale, RD 2015, 'SseK3 is a Salmonella effector that binds TRIM32 and modulates the host's NF-κB signalling activity', PLoS ONE, vol. 10, no. 9, e0138529. https://doi.org/10.1371/journal.pone.0138529

SseK3 is a Salmonella effector that binds TRIM32 and modulates the host's NF-κB signalling activity. / Yang, Zhe; Soderholm, Amelia; Lung, Tania Wong Fok; Giogha, Cristina; Hill, Michelle; Brown, Nathaniel F.; Hartland, Elizabeth; Teasdale, Rohan D.

In: PLoS ONE, Vol. 10, No. 9, e0138529, 22.09.2015.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Soderholm, Amelia

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