Projects per year
Methods: Spray-dried influenza vaccine was produced using previously established spray-drying conditions. The formulations were characterized to examine the impact of influenza antigen on the solid-state properties of the spray-dried powders. The optimal vaccine formulation was then selected for in vivo immunogenicity study in rats to evaluate the efficacy of the reconstituted spray-dried vaccine compared to liquid vaccine administered via pulmonary and subcutaneous routes.
Results: The formation of amorphous glassy matrix and morphology of the spray-dried particles, within the protein concentration range used in the study, was not affected by the incorporation of the influenza antigen. However, the amount of proteins incorporated increased water content and reduced the glass transition temperature (Tg) of the formulation. Nevertheless, the spray-dried vaccine induced strong mucosal and systemic immunity comparable to liquid vaccine after pulmonary and subcutaneous immunization without causing any inflammation to the lung parenchyma.
Conclusions: The study demonstrated the usability of the spray-dried carrier as a promising platform for pulmonary delivery of influenza vaccine. The potential utility of this delivery system for other biomacromolecules may also be further explored.
|Number of pages||11|
|Journal||Journal of Aerosol Medicine and Pulmonary Drug Delivery|
|Publication status||Published - 2015|
- influenza vaccine
- mucosal vaccine
- pulmonary delivery
- spray-drying; trehalose
- 3 Finished
Kaminskas, L., Bulitta, J. & Porter, C.
1/01/13 → 31/12/16
Administration of nanomedicines via the lungs to improve the treatment of primary and secondary lung cancers
1/01/12 → 31/12/15