Spontaneous electrical and Ca2+ signals in the mouse renal pelvis that drive pyeloureteric peristalsis

1. Peristalsis in the smooth muscle cell (SMC) wall of the pyeloureteric system is unique in physiology in that the primary pacemaker resides in a population of atypical SMCs situated near the border of the renal papilla. 2. Atypical SMCs display high frequency Ca(2+) transients upon the spontaneous release of Ca(2+) from IP(3)-dependent stores that trigger cation-selective spontaneous transient depolarizations (STDs). In nifedipine, these Ca(2+) transients and STDS seldom propagate >100 mum. Synchronization of STDs in neighbouring atypical SMCs into an electrical signal that can trigger action potential discharge and contraction in the typical SMC layer involves a coupled oscillator mechanism dependent on Ca(2+) entry through L-type voltage-operated Ca(2+) channels. 3. A population of spindle- or stellate-shaped cells, immuno-positive for the tyrosine receptor kinase kit, are sparsely distributed throughout the pyeloureteric system. Ca(2+) transients and action potentials of long duration occurring at low frequencies are also recorded in a population of fusiform cells, which we have termed interstitial cells of Cajal-like cells (ICC-like cells). 4. The electrical and Ca(2+) signals in ICC-like cells are abolished upon blockade of Ca(2+) release from either IP(3)- or ryanodine-dependent Ca(2+) stores. However, the spontaneous Ca(2+) signals in atypical SMCs or ICC-like cells are little affected in W/W(-v) transgenic mice which have extensive lesions of their intestinal ICC networks. 5. In summary, we have developed a model of pyeloureteric pacemaking in which atypical SMCs are indeed the primary pacemakers, the function of ICC-like cells has yet to be determined.