Sperm protein 17 expression by murine epithelial ovarian cancer cells and its impact on tumor progression

Qian Gao, Sue D. Xiang, Kirsty Wilson, Mutsa Madondo, Andrew N. Stephens, Magdalena Plebanski

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4 Citations (Scopus)

Abstract

The cancer testis antigen sperm protein 17 (Sp17) is a promising antigenic target in epithelial ovarian cancer (EOC) vaccine development. However, its role in ovarian cancer is unclear. We isolated and expanded Sp17+ and Sp17− clones from the murine EOC cell line ID8, and compared their in-vitro cell growth characteristics and in-vivo tumorigenicity. We also examined the potential co-expression of molecules that may influence cancer cell survival and interaction with immune cells. These include stimulatory and immunosuppressive molecules, such as major histocompatibility class I molecules (MHC I), MHC II, cytotoxic T lymphocyte associated antigen-4 (CTLA-4), CD73, CD39, tumor necrosis factor receptor II (TNFRII), signal transducer and activator of transcription 3 (STAT3) and programmed death-ligand 1 (PD-L1). Whilst the presence of Sp17 was not correlated with the ID8 cell proliferation/growth capacity in vitro, it was critical to enable progressive tumor formation in vivo. Flow cytometry revealed that Sp17+ ID8 cells displayed higher expression of both STAT3 and PD-L1, whilst MHC II expression was lower. Moreover, Sp17high (PD-L1+ MHCII−) cell populations showed significantly enhanced resistance to Paclitaxel-induced cell death in vitro compared to Sp17low (PD-L1− MHCII+) cells, which was associated in turn with increased STAT3 expression. Together, the data support Sp17 as a factor associated with in-vivo tumor progression and chemo-resistance, validating it as a suitable target for vaccine development.

Original languageEnglish
Article number276
Number of pages15
JournalCancers
Volume10
Issue number8
DOIs
Publication statusPublished - 20 Aug 2018

Keywords

  • ID8
  • Immune evasion
  • MHC II
  • Paclitaxel
  • PD-l1
  • Sperm protein 17 (Sp17)
  • STAT3
  • Tumor resistant

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