Spectroscopic signature of mouse embryonic stem cell-derived hepatocytes using synchrotron Fourier transform infrared microspectroscopy

Kanjana Thumanu, Waraporn Tanthanuch, Danna Ye, Anawat Sangmalee, Chanchao Lorthongpanich, Rangsun Parnpai, Philip Heraud

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Abstract

Stem cell-based therapy for liver regeneration has been proposed to overcome the persistent shortage in the supply of suitable donor organs. A requirement for this to succeed is to find a rapid method to detect functional hepatocytes, differentiated from embryonic stem cells. We propose Fourier transform infrared (FTIR) microspectroscopy as a versatile method to identify the early and last stages of the differentiation process leading to the formation of hepatocytes. Using synchrotron-FTIR microspectroscopy, the means of identifying hepatocytes at the single-cell level is possible and explored. Principal component analysis and subsequent partial leastsquares (PLS) discriminant analysis is applied to distinguish endoderm induction from hepatic progenitor cells and matured hepatocyte-like cells. The data are well modeled by PLS with endoderm induction, hepatic progenitor cells, and mature hepatocyte-like cells able to be discriminated with very high sensitivity and specificity. This method provides a practical tool to monitor endoderm induction and has the potential to be applied for quality control of cell differentiation leading to hepatocyte formation.

Original languageEnglish
Article number057005
JournalJournal of Biomedical Optics
Volume16
Issue number5
DOIs
Publication statusPublished - May 2011

Keywords

  • Fourier transform infrared microspectroscopy
  • Hepatocytes
  • Stem cells

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