Specificity of an esterase (XYLD) from Pseudomonas fluorescens subsp. cellulosa

Craig B. Faulds, Marie Christine Ralet, Gary Williamson, Geoffrey P. Hazlewood, Harry J. Gilbert

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36 Citations (Scopus)

Abstract

Activity of an esterase from Pseudomonas fluorescens subsp. cellulosa (XYLD) on an insoluble feruloylated hemicellulose substrate (de-starched wheat bran) was dependent on the source of added endo-xylanase. The esterase exhibited high selectivity for the nature, position of linkage and size of the feruloylated oligosaccharides generated by hydrolysis of the hemicellulose. Increased affinity of XYLD with increasing size of the oligosaccharide substrate suggests that optimal activity is observed on substrates with at least 4 sugars.

Original languageEnglish
Pages (from-to)265-269
Number of pages5
JournalBBA - General Subjects
Volume1243
Issue number2
DOIs
Publication statusPublished - 23 Feb 1995
Externally publishedYes

Keywords

  • (Pesudomonas)
  • Esterase
  • Ferulic acid
  • Oligosaccharide

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