Specific reactivity of recombinant human PDC-E1α in primary biliary cirrhosis

T. Iwayama, P. S.C. Leung, R. L. Coppel, T. E. Roche, M. S. Patel, Y. Mizushima, T. Nakagawa, R. Dickson, M. E. Gershwin

Research output: Contribution to journalArticleResearchpeer-review

21 Citations (Scopus)

Abstract

The mitochondrial autoantigens recognized by autoantibodies in patients with primary biliary cirrhosis have been identified as components of related multi-enzyme complexes, including acyltransferases of the pyruvate dehydrogenase complex (PDC), the branched-chain α-keto acid dehydrogenase complex (BCODH), the α-ketoglutarate dehydrogenase complex (OGDC), protein X and pyruvate dehydrogenase (PDC) E1α and E1β. The major autoantigens, PDC-E2, BCODH-E2 and OGDC-E2, share some sequence homology; the epitopes on these antigens appear to be close to, or identical with, the lipoic acid binding site. Furthermore, all three antigens share some structural homology. In contrast, antibodies to PDC-E1α are present in lower titers, and have been more difficult to detect. PDC-E1α also differs from the three major autoantigens in that it lacks any covalently bound lipoic acid. PDC-E1α cannot be purified in large quantities and becomes unstable in the absence of PDC-E1β. To address these problems, we have subcloned recombinant human PDC-E1α to pGEX. pGEX is a vector which produces a recombinant polypeptide fused to glutathione S-transferase. The resultant E1α fusion protein is stable and has a low background in immunoassays. Using the recombinant protein, we have developed an ELISA that allows rapid and reproducible quantification of antibodies to human PDC-E1α. Finally, we demonstrate that a major epitope on PDC-E1α is within a 300 amino acid region that contains the enzyme functional sites, namely the phosphorylation site and the TPP binding site.

Original languageEnglish
Pages (from-to)769-778
Number of pages10
JournalJournal of Autoimmunity
Volume4
Issue number5
DOIs
Publication statusPublished - 1 Jan 1991
Externally publishedYes

Cite this