Specific humoral response of hosts with variable schistosomiasis susceptibility

Patrick Driguez, Hamish EG McWilliam, Soraya Gaze, David Piedrafita, Mark S Pearson, Rie Nakajima, Mary Duke, Angela Trieu, Denise L Doolan, Fernanda C Cardoso, Algis Jasinskas, Geoffrey N Gobert, Philip L Felgner, Alex Loukas, Els Meeusen, Donald P McManus

Research output: Contribution to journalArticleResearchpeer-review

7 Citations (Scopus)

Abstract

The schistosome blood flukes are one of the largest global causes of parasitic morbidity. Further study of the specific antibody response during schistosomiasis may yield the vaccines and diagnostics needed to combat this disease. Therefore, for the purposes of antigen discovery, sera and antibody secreting cell (ASC) probes from semi-permissive rats and sera from susceptible mice were used to screen a schistosome protein microarray. Following Schistosoma japonicum infection, rats had reduced pathology, increased antibody responses and broader antigen recognition profiles compared with mice. With successive infections, rat global serological reactivity and the number of recognized antigens increased. The local antibody response in rat skin and lung, measured with ASC probes, increased after parasite migration and contributed antigen-specific antibodies to the multivalent serological response. In addition, the temporal variation of anti-parasite serum antibodies after infection and reinfection followed patterns that appear related to the antigen driving the response. Among the 29 antigens differentially recognized by the infected hosts, were numerous known vaccine candidates, drug targets, and several S. japonicum homologues of human schistosomiasis resistance markers-the tegument allergen-like (TAL) proteins. From this set we prioritized eight proteins that may prove to be novel schistosome vaccine and diagnostic antigens.
Original languageEnglish
Pages (from-to)52-65
Number of pages14
JournalImmunology and Cell Biology
Volume94
Issue number1
DOIs
Publication statusPublished - Jan 2016

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