Specific expression of GPR56 by human cytotoxic lymphocytes

Yen Ming Peng, Martijn D B van de Garde, Kai Fong Cheng, Paul A. Baars, Ester B M Remmerswaal, René A W van Lier, Charles R. Mackay, Hsi Hsien Lin, Jörg Hamann

Research output: Contribution to journalArticleResearchpeer-review

38 Citations (Scopus)

Abstract

We here report the existence of a new cluster of adhesion-GPCRs in human immune cells. Analysis of a comprehensive immune cell transcriptome dataset indicated that expression of the closely related receptors, GPR56, GPR97, and GPR114, is associated with single lymphocyte and granulocyte subsets. Applying flow cytometric analysis with newly generated mAb, we show that expression of GPR56 is restricted to cytotoxic NK and T lymphocytes, including CD8 +, CD4 +, and γδ T cells. Primary infection with human CMV, which generates a vast population of CD8 + T cells with an effector phenotype, induced a strong increase in GPR56 expression in virus-specific CD8 + T cells that remained detectable during latency. In NK-92 cells, ectopic expression of GPR56 inhibited spontaneous and SDF-1- stimulated cell migration. Our data suggest that GPR56 expression is a common trait of human cytotoxic lymphocytesand might affect the migratory properties of these cells.

Original languageEnglish
Pages (from-to)735-740
Number of pages6
JournalJournal of leukocyte biology
Volume90
Issue number4
DOIs
Publication statusPublished - Oct 2011
Externally publishedYes

Keywords

  • Adhesion-GPCRs
  • CMV
  • CTL
  • Migration
  • NK cells

Cite this

Peng, Y. M., van de Garde, M. D. B., Cheng, K. F., Baars, P. A., Remmerswaal, E. B. M., van Lier, R. A. W., ... Hamann, J. (2011). Specific expression of GPR56 by human cytotoxic lymphocytes. Journal of leukocyte biology, 90(4), 735-740. https://doi.org/10.1189/jlb.0211092
Peng, Yen Ming ; van de Garde, Martijn D B ; Cheng, Kai Fong ; Baars, Paul A. ; Remmerswaal, Ester B M ; van Lier, René A W ; Mackay, Charles R. ; Lin, Hsi Hsien ; Hamann, Jörg. / Specific expression of GPR56 by human cytotoxic lymphocytes. In: Journal of leukocyte biology. 2011 ; Vol. 90, No. 4. pp. 735-740.
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Peng, YM, van de Garde, MDB, Cheng, KF, Baars, PA, Remmerswaal, EBM, van Lier, RAW, Mackay, CR, Lin, HH & Hamann, J 2011, 'Specific expression of GPR56 by human cytotoxic lymphocytes', Journal of leukocyte biology, vol. 90, no. 4, pp. 735-740. https://doi.org/10.1189/jlb.0211092

Specific expression of GPR56 by human cytotoxic lymphocytes. / Peng, Yen Ming; van de Garde, Martijn D B; Cheng, Kai Fong; Baars, Paul A.; Remmerswaal, Ester B M; van Lier, René A W; Mackay, Charles R.; Lin, Hsi Hsien; Hamann, Jörg.

In: Journal of leukocyte biology, Vol. 90, No. 4, 10.2011, p. 735-740.

Research output: Contribution to journalArticleResearchpeer-review

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Peng YM, van de Garde MDB, Cheng KF, Baars PA, Remmerswaal EBM, van Lier RAW et al. Specific expression of GPR56 by human cytotoxic lymphocytes. Journal of leukocyte biology. 2011 Oct;90(4):735-740. https://doi.org/10.1189/jlb.0211092