Species-specific regulation of t-PA and PAI-1 gene expression in human and rat astrocytes

Anna Tjarnlund Wolf, Karin Hultman, Fredrik Blomstrand, Michael Nilsson, Robert Lindsay Medcalf, Christina Jern

Research output: Contribution to journalArticleResearchpeer-review

Abstract

In recent years, the role and physiological regulation of the serine protease tissue-type plasminogen activator (t-PA and its inhibitors, including plasminogen activator inhibitor type-1 (PAI-1, in the brain have received much attention. However, as studies focusing these issues are difficult to perform in humans, a great majority of the studies conducted to date have utilized rodent in vivo and/or in vitro models. In view of the species-specific structural differences present in both the t-PA and the PAI-1 promoters, we have compared the response of these genes in astrocytes of rat and human origin. We reveal marked quantitative and qualitative species-specific differences in gene induction following treatment with various physiological and pathological stimuli. Thus, our findings are of importance for the interpretation of previous and future results related to t-PA and PAI-1 expression.
Original languageEnglish
Pages (from-to)113 - 118
Number of pages6
JournalGene Regulation and Systems Biology
Volume2014
Issue number8
DOIs
Publication statusPublished - 2014

Cite this

Tjarnlund Wolf, Anna ; Hultman, Karin ; Blomstrand, Fredrik ; Nilsson, Michael ; Medcalf, Robert Lindsay ; Jern, Christina. / Species-specific regulation of t-PA and PAI-1 gene expression in human and rat astrocytes. In: Gene Regulation and Systems Biology. 2014 ; Vol. 2014, No. 8. pp. 113 - 118.
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abstract = "In recent years, the role and physiological regulation of the serine protease tissue-type plasminogen activator (t-PA and its inhibitors, including plasminogen activator inhibitor type-1 (PAI-1, in the brain have received much attention. However, as studies focusing these issues are difficult to perform in humans, a great majority of the studies conducted to date have utilized rodent in vivo and/or in vitro models. In view of the species-specific structural differences present in both the t-PA and the PAI-1 promoters, we have compared the response of these genes in astrocytes of rat and human origin. We reveal marked quantitative and qualitative species-specific differences in gene induction following treatment with various physiological and pathological stimuli. Thus, our findings are of importance for the interpretation of previous and future results related to t-PA and PAI-1 expression.",
author = "{Tjarnlund Wolf}, Anna and Karin Hultman and Fredrik Blomstrand and Michael Nilsson and Medcalf, {Robert Lindsay} and Christina Jern",
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Species-specific regulation of t-PA and PAI-1 gene expression in human and rat astrocytes. / Tjarnlund Wolf, Anna; Hultman, Karin; Blomstrand, Fredrik; Nilsson, Michael; Medcalf, Robert Lindsay; Jern, Christina.

In: Gene Regulation and Systems Biology, Vol. 2014, No. 8, 2014, p. 113 - 118.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Species-specific regulation of t-PA and PAI-1 gene expression in human and rat astrocytes

AU - Tjarnlund Wolf, Anna

AU - Hultman, Karin

AU - Blomstrand, Fredrik

AU - Nilsson, Michael

AU - Medcalf, Robert Lindsay

AU - Jern, Christina

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AB - In recent years, the role and physiological regulation of the serine protease tissue-type plasminogen activator (t-PA and its inhibitors, including plasminogen activator inhibitor type-1 (PAI-1, in the brain have received much attention. However, as studies focusing these issues are difficult to perform in humans, a great majority of the studies conducted to date have utilized rodent in vivo and/or in vitro models. In view of the species-specific structural differences present in both the t-PA and the PAI-1 promoters, we have compared the response of these genes in astrocytes of rat and human origin. We reveal marked quantitative and qualitative species-specific differences in gene induction following treatment with various physiological and pathological stimuli. Thus, our findings are of importance for the interpretation of previous and future results related to t-PA and PAI-1 expression.

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