SPDB: a comprehensive resource and knowledgebase for proteomic data at the single-cell resolution

The single-cell proteomics enables the direct quantification of proteinãbundanceãt the single-cell resolution, providing valuable insights into cellular phenotypes be y ond what can be inferred from transcriptomeãnalysisãlone. However, insufficient large-scale integrated databases hinder researchers fromãccessingãnd exploring single-cell proteomics, impeding theãdvancement of this field. To fill this deficiency, we presentã comprehensive database, namely Single-cell Proteomic DataBase (SPDB, https:// scproteomicsdb.com/ ), for general single-cell proteomic data, includingãntibody-based or mass spectrometry-based single-cell proteomics. Equipped with stãndardized datã processãndã user-friendly web interfãce, SPDB pro vides unified data formats for convenient interaction with downstreamãnalysis,ãnd offers not only dataset-level butãlso protein-le v el data searchãnd exploration capabilities. To enable detailed exhibition of single-cell proteomic data, SPDBãlso providesã module for visualizing data from the perspectives of cell metãdatã or protein features. The current version of SPDB encompasses 133ãntibody-based single-cell proteomic datasets in v olving more than 300 million cellsãnd o v er 800 mark er / surfãce proteins,ãnd 10 mass spectrometry-based single-cell proteomic datasets in v olving more than 40 0 0 cellsãnd o v er 70 0 0 proteins. Ov erall, SPDB is en visioned to be exploredãsã useful resource that will facilitate the wider research communities by providing detailed insights into proteomics from the single-cell perspective.