Spatiotemporal expression patterns of doublesex and mab-3 related transcription factor 1 in the chicken developing gonads and Müllerian ducts

T. Omotehara, C. A. Smith, Y. Mantani, Y Kobayashi, A. Tatsumi, D. Nagahara, R. Hashimoto, T Hirano, Y. Umemura, Tomonari Yokoyama, H Kitagawa, N. Hoshi

Research output: Contribution to journalArticleResearchpeer-review

6 Citations (Scopus)

Abstract

Sex of birds is genetically determined by the inheritance of sex chromosomes (ZZ for male and ZW for female), and the Z-linked gene named doublesex and mab-3 related transcription factor 1 (DMRT1) is a candidate sex-determining gene in avian species. However, the mechanisms underlying sex determination in birds are not yet understood, and the expression patterns of the DMRT1 protein in urogenital tissues have not been identified. In the current study, we used immunohistochemistry to investigate the detailed expression patterns of the DMRT1 protein in the urogenital systems (including Müllerian ducts) in male and female chicken embryos throughout embryonic development. Gonadal somatic cells in the male indifferent gonads showed stronger expressions of DMRT1 compared with those in the female indifferent gonads well before the presumptive period of the sex determination, and Sertoli cells forming testicular cords expressed DMRT1 in the testes after sex determination. Germ cells expressed DMRT1 equally in males and females after sex determination. The expression was continuous in males, but in females it gradually disappeared from the germ cells in the central part of the cortex of the left ovary toward both edges. The DMRT1 was also detected in the tubal ridge, which is a precursor of the Müllerian duct, and at the mesenchyme and outermost coelomic epithelium of the Müllerian duct in both sexes. Strong expression was observed in the males, but it was restricted to coelomic epithelium after the regression of the duct started. Thus, we observed the detailed spatiotemporal expression patterns of DMRT1 in the developing chicken urogenital systems throughout embryonic development, suggesting its various roles in the development of urogenital tissues in the chicken embryo.

Original languageEnglish
Pages (from-to)953-958
Number of pages6
JournalBritish Poultry Science
Volume93
Issue number4
DOIs
Publication statusPublished - Apr 2014
Externally publishedYes

Keywords

  • Doublesex and mab-3 related transcription factor 1
  • Embryonic development
  • Immunohistochemistry
  • Sex determination and differentiation
  • Urogenital tissue

Cite this

Omotehara, T. ; Smith, C. A. ; Mantani, Y. ; Kobayashi, Y ; Tatsumi, A. ; Nagahara, D. ; Hashimoto, R. ; Hirano, T ; Umemura, Y. ; Yokoyama, Tomonari ; Kitagawa, H ; Hoshi, N. / Spatiotemporal expression patterns of doublesex and mab-3 related transcription factor 1 in the chicken developing gonads and Müllerian ducts. In: British Poultry Science. 2014 ; Vol. 93, No. 4. pp. 953-958.
@article{7929e94e62004fe0a38a30201ad731ca,
title = "Spatiotemporal expression patterns of doublesex and mab-3 related transcription factor 1 in the chicken developing gonads and M{\"u}llerian ducts",
abstract = "Sex of birds is genetically determined by the inheritance of sex chromosomes (ZZ for male and ZW for female), and the Z-linked gene named doublesex and mab-3 related transcription factor 1 (DMRT1) is a candidate sex-determining gene in avian species. However, the mechanisms underlying sex determination in birds are not yet understood, and the expression patterns of the DMRT1 protein in urogenital tissues have not been identified. In the current study, we used immunohistochemistry to investigate the detailed expression patterns of the DMRT1 protein in the urogenital systems (including M{\"u}llerian ducts) in male and female chicken embryos throughout embryonic development. Gonadal somatic cells in the male indifferent gonads showed stronger expressions of DMRT1 compared with those in the female indifferent gonads well before the presumptive period of the sex determination, and Sertoli cells forming testicular cords expressed DMRT1 in the testes after sex determination. Germ cells expressed DMRT1 equally in males and females after sex determination. The expression was continuous in males, but in females it gradually disappeared from the germ cells in the central part of the cortex of the left ovary toward both edges. The DMRT1 was also detected in the tubal ridge, which is a precursor of the M{\"u}llerian duct, and at the mesenchyme and outermost coelomic epithelium of the M{\"u}llerian duct in both sexes. Strong expression was observed in the males, but it was restricted to coelomic epithelium after the regression of the duct started. Thus, we observed the detailed spatiotemporal expression patterns of DMRT1 in the developing chicken urogenital systems throughout embryonic development, suggesting its various roles in the development of urogenital tissues in the chicken embryo.",
keywords = "Doublesex and mab-3 related transcription factor 1, Embryonic development, Immunohistochemistry, Sex determination and differentiation, Urogenital tissue",
author = "T. Omotehara and Smith, {C. A.} and Y. Mantani and Y Kobayashi and A. Tatsumi and D. Nagahara and R. Hashimoto and T Hirano and Y. Umemura and Tomonari Yokoyama and H Kitagawa and N. Hoshi",
year = "2014",
month = "4",
doi = "10.3382/ps.2013-03672",
language = "English",
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Omotehara, T, Smith, CA, Mantani, Y, Kobayashi, Y, Tatsumi, A, Nagahara, D, Hashimoto, R, Hirano, T, Umemura, Y, Yokoyama, T, Kitagawa, H & Hoshi, N 2014, 'Spatiotemporal expression patterns of doublesex and mab-3 related transcription factor 1 in the chicken developing gonads and Müllerian ducts', British Poultry Science, vol. 93, no. 4, pp. 953-958. https://doi.org/10.3382/ps.2013-03672

Spatiotemporal expression patterns of doublesex and mab-3 related transcription factor 1 in the chicken developing gonads and Müllerian ducts. / Omotehara, T.; Smith, C. A.; Mantani, Y.; Kobayashi, Y; Tatsumi, A.; Nagahara, D.; Hashimoto, R.; Hirano, T; Umemura, Y.; Yokoyama, Tomonari; Kitagawa, H; Hoshi, N.

In: British Poultry Science, Vol. 93, No. 4, 04.2014, p. 953-958.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Spatiotemporal expression patterns of doublesex and mab-3 related transcription factor 1 in the chicken developing gonads and Müllerian ducts

AU - Omotehara, T.

AU - Smith, C. A.

AU - Mantani, Y.

AU - Kobayashi, Y

AU - Tatsumi, A.

AU - Nagahara, D.

AU - Hashimoto, R.

AU - Hirano, T

AU - Umemura, Y.

AU - Yokoyama, Tomonari

AU - Kitagawa, H

AU - Hoshi, N.

PY - 2014/4

Y1 - 2014/4

N2 - Sex of birds is genetically determined by the inheritance of sex chromosomes (ZZ for male and ZW for female), and the Z-linked gene named doublesex and mab-3 related transcription factor 1 (DMRT1) is a candidate sex-determining gene in avian species. However, the mechanisms underlying sex determination in birds are not yet understood, and the expression patterns of the DMRT1 protein in urogenital tissues have not been identified. In the current study, we used immunohistochemistry to investigate the detailed expression patterns of the DMRT1 protein in the urogenital systems (including Müllerian ducts) in male and female chicken embryos throughout embryonic development. Gonadal somatic cells in the male indifferent gonads showed stronger expressions of DMRT1 compared with those in the female indifferent gonads well before the presumptive period of the sex determination, and Sertoli cells forming testicular cords expressed DMRT1 in the testes after sex determination. Germ cells expressed DMRT1 equally in males and females after sex determination. The expression was continuous in males, but in females it gradually disappeared from the germ cells in the central part of the cortex of the left ovary toward both edges. The DMRT1 was also detected in the tubal ridge, which is a precursor of the Müllerian duct, and at the mesenchyme and outermost coelomic epithelium of the Müllerian duct in both sexes. Strong expression was observed in the males, but it was restricted to coelomic epithelium after the regression of the duct started. Thus, we observed the detailed spatiotemporal expression patterns of DMRT1 in the developing chicken urogenital systems throughout embryonic development, suggesting its various roles in the development of urogenital tissues in the chicken embryo.

AB - Sex of birds is genetically determined by the inheritance of sex chromosomes (ZZ for male and ZW for female), and the Z-linked gene named doublesex and mab-3 related transcription factor 1 (DMRT1) is a candidate sex-determining gene in avian species. However, the mechanisms underlying sex determination in birds are not yet understood, and the expression patterns of the DMRT1 protein in urogenital tissues have not been identified. In the current study, we used immunohistochemistry to investigate the detailed expression patterns of the DMRT1 protein in the urogenital systems (including Müllerian ducts) in male and female chicken embryos throughout embryonic development. Gonadal somatic cells in the male indifferent gonads showed stronger expressions of DMRT1 compared with those in the female indifferent gonads well before the presumptive period of the sex determination, and Sertoli cells forming testicular cords expressed DMRT1 in the testes after sex determination. Germ cells expressed DMRT1 equally in males and females after sex determination. The expression was continuous in males, but in females it gradually disappeared from the germ cells in the central part of the cortex of the left ovary toward both edges. The DMRT1 was also detected in the tubal ridge, which is a precursor of the Müllerian duct, and at the mesenchyme and outermost coelomic epithelium of the Müllerian duct in both sexes. Strong expression was observed in the males, but it was restricted to coelomic epithelium after the regression of the duct started. Thus, we observed the detailed spatiotemporal expression patterns of DMRT1 in the developing chicken urogenital systems throughout embryonic development, suggesting its various roles in the development of urogenital tissues in the chicken embryo.

KW - Doublesex and mab-3 related transcription factor 1

KW - Embryonic development

KW - Immunohistochemistry

KW - Sex determination and differentiation

KW - Urogenital tissue

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U2 - 10.3382/ps.2013-03672

DO - 10.3382/ps.2013-03672

M3 - Article

VL - 93

SP - 953

EP - 958

JO - British Poultry Science

JF - British Poultry Science

SN - 0007-1668

IS - 4

ER -