Spatially controlled photochemical peptide and polymer conjugation on biosurfaces

Thomas Tischer, Tanja K. Claus, Michael Bruns, Vanessa Trouillet, Katharina Linkert, Cesar Rodriguez-Emmenegger, Anja S. Goldmann, Sebastien Perrier, Hans G. Börner, Christopher Barner-Kowollik

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35 Citations (Scopus)

Abstract

An efficient phototriggered Diels-Alder conjugation is utilized to graft in an effective and straightforward approach poly(trifluoro ethyl methacrylate) (Mn = 3700 Da, Crossed D sign = 1.27) and a model peptide (GIGKFLHS) onto thin hyaluronan films and cellulose surfaces. The surfaces were functionalized with an o-quinodimethane moiety - capable of releasing a caged diene - via carbodiimide mediated coupling. The o-quinodimethane group is employed as a photoactive linker to tether predefined peptide/polymer strands in a spatially controlled manner onto the biosurface by photoenol ligation. An in-depth characterization employing XPS, ToF-SIMS, SPR, ellipsometry, and AFM was conducted to evidence the effectiveness of the presented approach.

Original languageEnglish
Pages (from-to)4340-4350
Number of pages11
JournalBiomacromolecules
Volume14
Issue number12
DOIs
Publication statusPublished - 9 Dec 2013
Externally publishedYes

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