Spatial dissection of the cis- and trans-Golgi compartments in the malaria parasite Plasmodium falciparum

Nicole Struck, Susann Herrmann, Iris Schmuck-Barkmann, Suzana Dias, Silvia Haase, Ana L Cabrera, Moritz Treeck, Caroline Bruns, Christine Langer, Alan Frederick Cowman, Matthias Marti, Tobias Spielmann, Tim W Gilberger

Research output: Contribution to journalArticleOther

30 Citations (Scopus)

Abstract

The Golgi apparatus forms the heart of the secretory pathway in eukaryotic cells where proteins are modified, processed and sorted. The transport of proteins from the endoplasmic reticulum (ER) to the cis-side of the Golgi complex takes place at specialized ER sub-domains known as transitional ER (tER). We used the Plasmodium falciparum orthologue of Sec13p to analyse tER organization. We show that the distribution of PfSec13p is restricted to defined areas of the ER membrane. These foci are juxtaposed to the Golgi apparatus and might represent tER sites. To further analyse cis- to trans-Golgi architecture, we generated a double transfectant parasite line that expresses the Golgi marker Golgi reassembly stacking protein (GRASP) as a green fluorescent protein fusion and the trans-Golgi marker Rab6 as a DsRed fusion protein. Our data demonstrate that Golgi multiplication is closely linked to tER multiplication, and that parasite maturation is accompanied by the spatial separation of the cis- and trans- face of this organelle.
Original languageEnglish
Pages (from-to)1320 - 1330
Number of pages11
JournalMolecular Microbiology
Volume67
Issue number6
DOIs
Publication statusPublished - 2008
Externally publishedYes

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