Spatial and temporal relationships between Shh, Fgf4, and Fgf8 gene expression at diverse signalling centers during mouse development

David Bueno, Judith Skinner, Helen Abud, John K. Heath

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31 Citations (Scopus)


During limb outgrowth, Shh, Fgf4, and Fgf8 act as signals controlling limb growth and patterning. Because these genes are expressed in the limb bud and other known signalling centers, we have explored the relationships between the expression of these genes during mouse development using double in situ hybridization. Within the node and limb bud the expression domains of these genes contact each other, whereas in the floor plate the Fgf8 expression domain does not contact that of Shh. The relative temporal order of gene expression varies in different centers. The spatial and temporal expression of Fgf4, Fgf8, and Shh suggests the conservation of molecular mechanisms in different organizing centers as well as differences between them.

Original languageEnglish
Pages (from-to)291-299
Number of pages9
JournalDevelopmental Dynamics
Issue number3
Publication statusPublished - Nov 1996
Externally publishedYes


  • Embryo patterning
  • Fibroblast growth factor
  • Limb development
  • Node
  • Signalling centers
  • Sonic hedgehog

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