To detect extremely small magnetic fields generated by the human brain, currently all commercial magnetoencephalography (MEG) systems are equipped with low-temperature (low-Tc) superconducting quantum interference device (SQUID) sensors that use liquid helium for cooling. The limited and increasingly expensive supply of helium, which has seen dramatic price increases recently, has become a real problem for such systems and the situation shows no signs of abating. MEG research in the long run is now endangered. In this study, we report a MEG source localization utilizing a single, highly sensitive SQUID cooled with liquid nitrogen only. Our findings confirm that localization of neuromagnetic activity is indeed possible using high-Tc SQUIDs. We believe that our findings secure the future of this exquisitely sensitive technique and have major implications for brain research and the developments of cost-effective multi-channel, high-Tc SQUID-based MEG systems.