TY - JOUR
T1 - Soluble inhibitors generated during hydrothermal pretreatment of oil palm mesocarp fiber suppressed the catalytic activity of Acremonium cellulase
AU - Zakaria, Mohd Rafein
AU - Hirata, Satoshi
AU - Fujimoto, Shinji
AU - Ibrahim, Izzudin
AU - Hassan, Mohd Ali
N1 - Funding Information:
This work was partly supported by the Science and Technology Research Partnership for Sustainable Development (SATREPS), under Japan Science and Technology Agency (JST) and Japan International Cooperation Agency (JICA) under leadership of Prof. Dr. Yoshihito Shirai from Kyushu Institute of Technology (Kyutech), Japan. Special thanks to Ms. Yuuki Nishimoto for the technical assistance.
Publisher Copyright:
© 2015 Elsevier Ltd.
PY - 2016/1/1
Y1 - 2016/1/1
N2 - Oil palm mesocarp fiber was subjected to hydrothermal pretreatment under isothermal and non-isothermal conditions. The pretreated slurries were separated by filtration, pretreated liquids and solids were characterized. An enzymatic digestibility study was performed for both pretreated slurries and solids to understand the effect of soluble inhibitors generated during the pretreatment process. The highest glucose yield obtained from pretreated slurries was 70.1%, and gradually decreased with higher pretreatment severities. The highest glucose yield obtained in pretreated solids was 100%, after pretreatment at 210. °C for 20. min. In order to study the inhibitory effects of compounds generated during pretreatment with cellulase, technical grade solutions that mimic the pretreated liquid were prepared and their effect on Acremonium cellulase activity was monitored using Avicel. Xylo-oligomers and tannic acid were identified as powerful inhibitors of Acremonium cellulase, and the lowest hydrolysis rate of Avicel of 0.18. g/g-glucose released/L/h was obtained from tannic acid.
AB - Oil palm mesocarp fiber was subjected to hydrothermal pretreatment under isothermal and non-isothermal conditions. The pretreated slurries were separated by filtration, pretreated liquids and solids were characterized. An enzymatic digestibility study was performed for both pretreated slurries and solids to understand the effect of soluble inhibitors generated during the pretreatment process. The highest glucose yield obtained from pretreated slurries was 70.1%, and gradually decreased with higher pretreatment severities. The highest glucose yield obtained in pretreated solids was 100%, after pretreatment at 210. °C for 20. min. In order to study the inhibitory effects of compounds generated during pretreatment with cellulase, technical grade solutions that mimic the pretreated liquid were prepared and their effect on Acremonium cellulase activity was monitored using Avicel. Xylo-oligomers and tannic acid were identified as powerful inhibitors of Acremonium cellulase, and the lowest hydrolysis rate of Avicel of 0.18. g/g-glucose released/L/h was obtained from tannic acid.
KW - Acremonium cellulase inhibition
KW - Hydrothermal pretreatment
KW - Oil palm mesocarp fiber
KW - Tannic acid
KW - Xylo-oligomers
UR - http://www.scopus.com/inward/record.url?scp=84945358040&partnerID=8YFLogxK
U2 - 10.1016/j.biortech.2015.10.075
DO - 10.1016/j.biortech.2015.10.075
M3 - Article
C2 - 26524253
AN - SCOPUS:84945358040
SN - 0960-8524
VL - 200
SP - 541
EP - 547
JO - Bioresource Technology
JF - Bioresource Technology
ER -
