Soluble factors derived from human amniotic epithelial cells suppress collagen production in human hepatic stellate cells

Alex Hodge, Dinushka Lourensz, Vijesh Vaghjiani, Kristy Huyen Nguyen, Jorge Tchongue, Bo Wang, Padma Murthi, William Sievert, Ursula Chandini Manuelpillai

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Background: Intravenous infusion of human amniotic epithelial cells (hAECs) has been shown to ameliorate hepatic fibrosis in murine models. Hepatic stellate cells (HSCs) are the principal collagen-secreting cells in the liver. The aim of this study was to investigate whether factors secreted by hAECs and present in hAEC-conditioned medium (CM) have anti-fibrotic effects on activated human HSCs. Methods: Human AECs were isolated from the placenta and cultured. Human hepatic stellate cells were exposed to hAEC CM to determine potential anti-fibrotic effects. Results: HSCs treated for 48 h with hAEC CM displayed a significant reduction in the expression of the myofibroblast markers a-smooth muscle actin and platelet-derived growth factor. Expression of the pro-fibrotic cytokine transforming growth factor-?1 (TGF-?1) and intracellular collagen were reduced by 45 and 46 , respectively. Human AEC CM induced HSC apoptosis in 11.8 of treated cells and reduced HSC proliferation. Soluble human leukocyte antigen-G1, a hAEC-derived factor, significantly decreased TGF-?1 and collagen production in activated HSCs, although the effect on collagen production was less than that of hAEC CM. The reduction in collagen and TGF-B1 could not be attributed to PGE2, relaxin, IL-10, TGF-B3, FasL or TRAIL. Conclusions: Human AEC CM treatment suppresses markers of activation, proliferation and fibrosis in human HSCs as well as inducing apoptosis and reducing proliferation
Original languageEnglish
Pages (from-to)1132 - 1144
Number of pages13
JournalCytotherapy
Volume16
Issue number8
DOIs
Publication statusPublished - 2014

Cite this

Hodge, A., Lourensz, D., Vaghjiani, V., Nguyen, K. H., Tchongue, J., Wang, B., ... Manuelpillai, U. C. (2014). Soluble factors derived from human amniotic epithelial cells suppress collagen production in human hepatic stellate cells. Cytotherapy, 16(8), 1132 - 1144. https://doi.org/10.1016/j.jcyt.2014.01.005
Hodge, Alex ; Lourensz, Dinushka ; Vaghjiani, Vijesh ; Nguyen, Kristy Huyen ; Tchongue, Jorge ; Wang, Bo ; Murthi, Padma ; Sievert, William ; Manuelpillai, Ursula Chandini. / Soluble factors derived from human amniotic epithelial cells suppress collagen production in human hepatic stellate cells. In: Cytotherapy. 2014 ; Vol. 16, No. 8. pp. 1132 - 1144.
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title = "Soluble factors derived from human amniotic epithelial cells suppress collagen production in human hepatic stellate cells",
abstract = "Background: Intravenous infusion of human amniotic epithelial cells (hAECs) has been shown to ameliorate hepatic fibrosis in murine models. Hepatic stellate cells (HSCs) are the principal collagen-secreting cells in the liver. The aim of this study was to investigate whether factors secreted by hAECs and present in hAEC-conditioned medium (CM) have anti-fibrotic effects on activated human HSCs. Methods: Human AECs were isolated from the placenta and cultured. Human hepatic stellate cells were exposed to hAEC CM to determine potential anti-fibrotic effects. Results: HSCs treated for 48 h with hAEC CM displayed a significant reduction in the expression of the myofibroblast markers a-smooth muscle actin and platelet-derived growth factor. Expression of the pro-fibrotic cytokine transforming growth factor-?1 (TGF-?1) and intracellular collagen were reduced by 45 and 46 , respectively. Human AEC CM induced HSC apoptosis in 11.8 of treated cells and reduced HSC proliferation. Soluble human leukocyte antigen-G1, a hAEC-derived factor, significantly decreased TGF-?1 and collagen production in activated HSCs, although the effect on collagen production was less than that of hAEC CM. The reduction in collagen and TGF-B1 could not be attributed to PGE2, relaxin, IL-10, TGF-B3, FasL or TRAIL. Conclusions: Human AEC CM treatment suppresses markers of activation, proliferation and fibrosis in human HSCs as well as inducing apoptosis and reducing proliferation",
author = "Alex Hodge and Dinushka Lourensz and Vijesh Vaghjiani and Nguyen, {Kristy Huyen} and Jorge Tchongue and Bo Wang and Padma Murthi and William Sievert and Manuelpillai, {Ursula Chandini}",
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Hodge, A, Lourensz, D, Vaghjiani, V, Nguyen, KH, Tchongue, J, Wang, B, Murthi, P, Sievert, W & Manuelpillai, UC 2014, 'Soluble factors derived from human amniotic epithelial cells suppress collagen production in human hepatic stellate cells' Cytotherapy, vol. 16, no. 8, pp. 1132 - 1144. https://doi.org/10.1016/j.jcyt.2014.01.005

Soluble factors derived from human amniotic epithelial cells suppress collagen production in human hepatic stellate cells. / Hodge, Alex; Lourensz, Dinushka; Vaghjiani, Vijesh; Nguyen, Kristy Huyen; Tchongue, Jorge; Wang, Bo; Murthi, Padma; Sievert, William; Manuelpillai, Ursula Chandini.

In: Cytotherapy, Vol. 16, No. 8, 2014, p. 1132 - 1144.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Soluble factors derived from human amniotic epithelial cells suppress collagen production in human hepatic stellate cells

AU - Hodge, Alex

AU - Lourensz, Dinushka

AU - Vaghjiani, Vijesh

AU - Nguyen, Kristy Huyen

AU - Tchongue, Jorge

AU - Wang, Bo

AU - Murthi, Padma

AU - Sievert, William

AU - Manuelpillai, Ursula Chandini

PY - 2014

Y1 - 2014

N2 - Background: Intravenous infusion of human amniotic epithelial cells (hAECs) has been shown to ameliorate hepatic fibrosis in murine models. Hepatic stellate cells (HSCs) are the principal collagen-secreting cells in the liver. The aim of this study was to investigate whether factors secreted by hAECs and present in hAEC-conditioned medium (CM) have anti-fibrotic effects on activated human HSCs. Methods: Human AECs were isolated from the placenta and cultured. Human hepatic stellate cells were exposed to hAEC CM to determine potential anti-fibrotic effects. Results: HSCs treated for 48 h with hAEC CM displayed a significant reduction in the expression of the myofibroblast markers a-smooth muscle actin and platelet-derived growth factor. Expression of the pro-fibrotic cytokine transforming growth factor-?1 (TGF-?1) and intracellular collagen were reduced by 45 and 46 , respectively. Human AEC CM induced HSC apoptosis in 11.8 of treated cells and reduced HSC proliferation. Soluble human leukocyte antigen-G1, a hAEC-derived factor, significantly decreased TGF-?1 and collagen production in activated HSCs, although the effect on collagen production was less than that of hAEC CM. The reduction in collagen and TGF-B1 could not be attributed to PGE2, relaxin, IL-10, TGF-B3, FasL or TRAIL. Conclusions: Human AEC CM treatment suppresses markers of activation, proliferation and fibrosis in human HSCs as well as inducing apoptosis and reducing proliferation

AB - Background: Intravenous infusion of human amniotic epithelial cells (hAECs) has been shown to ameliorate hepatic fibrosis in murine models. Hepatic stellate cells (HSCs) are the principal collagen-secreting cells in the liver. The aim of this study was to investigate whether factors secreted by hAECs and present in hAEC-conditioned medium (CM) have anti-fibrotic effects on activated human HSCs. Methods: Human AECs were isolated from the placenta and cultured. Human hepatic stellate cells were exposed to hAEC CM to determine potential anti-fibrotic effects. Results: HSCs treated for 48 h with hAEC CM displayed a significant reduction in the expression of the myofibroblast markers a-smooth muscle actin and platelet-derived growth factor. Expression of the pro-fibrotic cytokine transforming growth factor-?1 (TGF-?1) and intracellular collagen were reduced by 45 and 46 , respectively. Human AEC CM induced HSC apoptosis in 11.8 of treated cells and reduced HSC proliferation. Soluble human leukocyte antigen-G1, a hAEC-derived factor, significantly decreased TGF-?1 and collagen production in activated HSCs, although the effect on collagen production was less than that of hAEC CM. The reduction in collagen and TGF-B1 could not be attributed to PGE2, relaxin, IL-10, TGF-B3, FasL or TRAIL. Conclusions: Human AEC CM treatment suppresses markers of activation, proliferation and fibrosis in human HSCs as well as inducing apoptosis and reducing proliferation

UR - http://www.sciencedirect.com/science/article/pii/S1465324914000255

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DO - 10.1016/j.jcyt.2014.01.005

M3 - Article

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SP - 1132

EP - 1144

JO - Cytotherapy

JF - Cytotherapy

SN - 1465-3249

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