Solubilisation partial characterisation of the alpha-MSH receptor on primary rat Schwann cells

Jason K. Dyer, Abdel R.H. Ahmed, George W.J. Oliver, C. W. Pouton, Laurence W. Haynes

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The ACTH/MSH melanocortin core peptide sequence possesses neurotrophic properties in peripheral nerve. During functional neuroanatomical recovery after damage to peripheral nerves, Schwann cells play a significant role in facilitating regeneration. Here we employ a modified super-potent alpha-MSH analogue to solubilise alpha-MSH receptor proteins from cultured primary rat Schwann cells. [125I-Tyr2,Nle4,D-Phe7,ATB-Lys11]-alpha-MSH photoaffinity labelled proteins from Schwann cells were analyzed by SDS-PAGE followed by autoradiography. The results indicate that the alpha-MSH receptor proteins labelled have a molecular weight of 42-45 kDa. These data are the first to demonstrate solubilisation and characterisation of alpha-MSH receptors from non-melanoma cells.
Original languageEnglish
Pages (from-to)103-106
Number of pages4
JournalFEBS Letters
Volume336
DOIs
Publication statusPublished - 1993
Externally publishedYes

Keywords

  • Amino Acid Sequence Animals Autoradiography Electrophoresis, Polyacrylamide Gel Female Male Molecular Sequence Data Rats Rats, Wistar Receptors, Pituitary Hormone/*metabolism Schwann Cells/*metabolism Solubility alpha-MSH/metabolism

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