Snapshot prediction of carbon productivity, carbon and protein content in a Southern Ocean diatom using FTIR spectroscopy

Olivia Sackett, Katherina Petrou, Brian Reedy, Ross Hill, Martina A Doblin, John Beardall, Peter J Ralph, Philip Robert Heraud

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Diatoms, an important group of phytoplankton, bloom annually in the Southern Ocean, covering thousands of square kilometers and dominating the region's phytoplankton communities. In their role as the major food source to marine grazers, diatoms supply carbon, nutrients and energy to the Southern Ocean food web. Prevailing environmental conditions influence diatom phenotypic traits (for example, photophysiology, macromolecular composition and morphology), which in turn affect the transfer of energy, carbon and nutrients to grazers and higher trophic levels, as well as oceanic biogeochemical cycles. The paucity of phenotypic data on Southern Ocean phytoplankton limits our understanding of the ecosystem and how it may respond to future environmental change. Here we used a novel approach to create a 'snapshot' of cell phenotype. Using mass spectrometry, we measured nitrogen (a proxy for protein), total carbon and carbon-13 enrichment (carbon productivity), then used this data to build spectroscopy-based predictive models. The models were used to provide phenotypic data for samples from a third sample set. Importantly, this approach enabled the first ever rate determination of carbon productivity from a single time point, circumventing the need for time-series measurements. This study showed that Chaetoceros simplex was less productive and had lower protein and carbon content during short-term periods of high salinity. Applying this new phenomics approach to natural phytoplankton samples could provide valuable insight into understanding phytoplankton productivity and function in the marine system.
Original languageEnglish
Pages (from-to)416-426
Number of pages11
JournalISME Journal
Volume10
Issue number2
DOIs
Publication statusPublished - 2016

Cite this

Sackett, Olivia ; Petrou, Katherina ; Reedy, Brian ; Hill, Ross ; Doblin, Martina A ; Beardall, John ; Ralph, Peter J ; Heraud, Philip Robert. / Snapshot prediction of carbon productivity, carbon and protein content in a Southern Ocean diatom using FTIR spectroscopy. In: ISME Journal. 2016 ; Vol. 10, No. 2. pp. 416-426.
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abstract = "Diatoms, an important group of phytoplankton, bloom annually in the Southern Ocean, covering thousands of square kilometers and dominating the region's phytoplankton communities. In their role as the major food source to marine grazers, diatoms supply carbon, nutrients and energy to the Southern Ocean food web. Prevailing environmental conditions influence diatom phenotypic traits (for example, photophysiology, macromolecular composition and morphology), which in turn affect the transfer of energy, carbon and nutrients to grazers and higher trophic levels, as well as oceanic biogeochemical cycles. The paucity of phenotypic data on Southern Ocean phytoplankton limits our understanding of the ecosystem and how it may respond to future environmental change. Here we used a novel approach to create a 'snapshot' of cell phenotype. Using mass spectrometry, we measured nitrogen (a proxy for protein), total carbon and carbon-13 enrichment (carbon productivity), then used this data to build spectroscopy-based predictive models. The models were used to provide phenotypic data for samples from a third sample set. Importantly, this approach enabled the first ever rate determination of carbon productivity from a single time point, circumventing the need for time-series measurements. This study showed that Chaetoceros simplex was less productive and had lower protein and carbon content during short-term periods of high salinity. Applying this new phenomics approach to natural phytoplankton samples could provide valuable insight into understanding phytoplankton productivity and function in the marine system.",
author = "Olivia Sackett and Katherina Petrou and Brian Reedy and Ross Hill and Doblin, {Martina A} and John Beardall and Ralph, {Peter J} and Heraud, {Philip Robert}",
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Snapshot prediction of carbon productivity, carbon and protein content in a Southern Ocean diatom using FTIR spectroscopy. / Sackett, Olivia; Petrou, Katherina; Reedy, Brian; Hill, Ross; Doblin, Martina A; Beardall, John; Ralph, Peter J; Heraud, Philip Robert.

In: ISME Journal, Vol. 10, No. 2, 2016, p. 416-426.

Research output: Contribution to journalArticleResearchpeer-review

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