Smouse testis pdha-2 promoter upstream sequences confer tissue- and temporal-specific activity in transgenic mice

Rocco C. Iannello, Julia C. Young, Sony Sumarsono, Martin J. Tymms, Ismail Kola

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7 Citations (Scopus)


Spermatogenesis is a complex process requiring the coordinate expression of a number of testis-specific genes. One of these, Pdha-2, codes for the murine testis-specific isoform of the Ela subunit of the pyruvate dehydrogenase complex. To elucidate the mechanisms regulating its expression in vivo, we have begun to investigate the Pdha-2 promoter in transgenic mice. In this paper, a construct containing 3 • 0 kb of promoter and upstream sequences is reported to be sufficient for directing the testis-specific expression of a CAT reporter gene in mice harbouring the transgene. Similarly to the endogenous Pdha-2, the CAT gene is expressed in testis in a stage-specific manner. However, the 3-0-kb Pdha-2 promoter is not active in somatic tissue suggesting that repressor elements may be present within these sequences.

Original languageEnglish
Pages (from-to)599-604
Number of pages6
JournalReproduction, Fertility and Development
Issue number5
Publication statusPublished - 1 Jan 1994
Externally publishedYes


  • Mouse
  • Spermatogenesis
  • Transcription factors

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