Skin dendritic cell and T cell activation associated with dengue shock syndrome

Huynh Thi Le Duyen, Daniela Cerny, Dinh The Trung, Jassia Pang, Sumathy Velumani, Ying Xiu Toh, Phan Tu Qui, Nguyen Van Hao, Cameron Simmons, Muzlifah Haniffa, Bridget Wills, Katja Fink

Research output: Contribution to journalArticleResearchpeer-review

9 Citations (Scopus)

Abstract

The pathogenesis of severe dengue remains unclear, particularly the mechanisms underlying the plasma leakage that results in hypovolaemic shock in a small proportion of individuals. Maximal leakage occurs several days after peak viraemia implicating immunological pathways. Skin is a highly vascular organ and also an important site of immune reactions with a high density of dendritic cells (DCs), macrophages and T cells. We obtained skin biopsies and contemporaneous blood samples from patients within 24 hours of onset of dengue shock syndrome (DSS), and from healthy controls. We analyzed cell subsets by flow cytometry, and soluble mediators and antibodies by ELISA; the percentage of migratory CD1a+ dermal DCs was significantly decreased in the DSS patients, and skin CD8+ T cells were activated, but there was no accumulation of dengue-specific antibodies. Inflammatory monocytic cells were not observed infiltrating the skin of DSS cases on whole-mount histology, although CD14dim cells disappeared from blood.

Original languageEnglish
Article number14224
Number of pages12
JournalScientific Reports
Volume7
Issue number1
DOIs
Publication statusPublished - 1 Dec 2017
Externally publishedYes

Cite this