Site-Specific Cryo-focused Ion Beam Sample Preparation Guided by 3D Correlative Microscopy

Jan Arnold, Julia Mahamid, Vladan Lucic, Alex De Marco, Jose Jesus Fernandez, Tim Laugks, Tobias Mayer, Anthony A. Hyman, Wolfgang Baumeister , Jürgen M. Plitzko

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50 Citations (Scopus)

Abstract

The development of cryo-focused ion beam (cryo-FIB) for the thinning of frozen-hydrated biological specimens enabled cryo-electron tomography (cryo-ET) analysis in unperturbed cells and tissues. However, the volume represented within a typical FIB lamella constitutes a small fraction of the biological specimen. Retaining low-abundance and dynamic subcellular structures or macromolecular assemblies within such limited volumes requires precise targeting of the FIB milling process. In this study, we present the development of a cryo-stage allowing for spinning-disk confocal light microscopy at cryogenic temperatures and describe the incorporation of the new hardware into existing workflows for cellular sample preparation by cryo-FIB. Introduction of fiducial markers and subsequent computation of three-dimensional coordinate transformations provide correlation between light microscopy and scanning electron microscopy/FIB. The correlative approach is employed to guide the FIB milling process of vitrified cellular samples and to capture specific structures, namely fluorescently labeled lipid droplets, in lamellas that are 300 nm thick. The correlation procedure is then applied to localize the fluorescently labeled structures in the transmission electron microscopy image of the lamella. This approach can be employed to navigate the acquisition of cryo-ET data within FIB-lamellas at specific locations, unambiguously identified by fluorescence microscopy.

Original languageEnglish
Pages (from-to)860-869
Number of pages10
JournalBiophysical Journal
Volume110
Issue number4
DOIs
Publication statusPublished - 23 Feb 2016

Cite this

Arnold, J., Mahamid, J., Lucic, V., De Marco, A., Fernandez, J. J., Laugks, T., ... Plitzko, J. M. (2016). Site-Specific Cryo-focused Ion Beam Sample Preparation Guided by 3D Correlative Microscopy. Biophysical Journal, 110(4), 860-869. https://doi.org/10.1016/j.bpj.2015.10.053
Arnold, Jan ; Mahamid, Julia ; Lucic, Vladan ; De Marco, Alex ; Fernandez, Jose Jesus ; Laugks, Tim ; Mayer, Tobias ; Hyman, Anthony A. ; Baumeister , Wolfgang ; Plitzko, Jürgen M. / Site-Specific Cryo-focused Ion Beam Sample Preparation Guided by 3D Correlative Microscopy. In: Biophysical Journal. 2016 ; Vol. 110, No. 4. pp. 860-869.
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Arnold, J, Mahamid, J, Lucic, V, De Marco, A, Fernandez, JJ, Laugks, T, Mayer, T, Hyman, AA, Baumeister , W & Plitzko, JM 2016, 'Site-Specific Cryo-focused Ion Beam Sample Preparation Guided by 3D Correlative Microscopy', Biophysical Journal, vol. 110, no. 4, pp. 860-869. https://doi.org/10.1016/j.bpj.2015.10.053

Site-Specific Cryo-focused Ion Beam Sample Preparation Guided by 3D Correlative Microscopy. / Arnold, Jan; Mahamid, Julia; Lucic, Vladan; De Marco, Alex; Fernandez, Jose Jesus; Laugks, Tim; Mayer, Tobias; Hyman, Anthony A.; Baumeister , Wolfgang; Plitzko, Jürgen M.

In: Biophysical Journal, Vol. 110, No. 4, 23.02.2016, p. 860-869.

Research output: Contribution to journalArticleResearchpeer-review

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