Single-step microfluidic synthesis of transferrin-conjugated lipid nanoparticles for siRNA delivery

Yujing Li, Robert J. Lee, Xueqin Huang, Yuhuan Li, Bingcong Lv, Tianpeng Wang, Yuhang Qi, Fei Hao, Jiahui Lu, Qingfan Meng, Lirong Teng, Yulin Zhou, Jing-JIng Xie, Lesheng Teng

Research output: Contribution to journalArticleResearchpeer-review

23 Citations (Scopus)


Microfluidic systems can accelerate clinical translation of nanoparticles due to their ability to generate nanoparticles in a well-controlled and reproducible manner. In this study, a single-step process based on microfluidic focusing (MF) was employed to synthesize transferrin-conjugated lipid nanoparticles (Tf-LNPs) and the method was compared with a multi-steps bulk mixing (BM) method. The results indicate that this single-step MF process enables rapid and efficient synthesis of Tf-LNPs, which were named Tf-LNPs-MF. Tf-LNPs-MF was shown to have a smaller size and more uniform structures compared to LNPs produced by multi-steps BM method (Tf-LNPs-BM). Furthermore, efficient cellular uptake of Tf-LNPs-MF in vitro as well as greater tumor inhibition in vivo proved that Tf-LNPs-MF had higher siRNA delivery efficiency in vitro and in vivo. Taken together, this single-step microfluidic synthesis significantly simplified the Tf-LNPs production and improved their drug delivery properties and may serve as a valuable tool for developing new cancer therapies.

Original languageEnglish
Pages (from-to)371-381
Number of pages11
JournalNanomedicine-Nanotechnology Biology and Medicine
Issue number2
Publication statusPublished - 1 Feb 2017
Externally publishedYes


  • Cancer
  • Lipid nanoparticle
  • Microfluidics
  • siRNA
  • Transferrin

Cite this