TY - JOUR
T1 - Single-molecule DNA flexibility in the presence of base-pair mismatch
AU - Schallhorn, Kathryn A.
AU - Freedman, Katherine O.
AU - Moore, Jessica M.
AU - Lin, Jun
AU - Ke, Pu Chun
PY - 2005/7/18
Y1 - 2005/7/18
N2 - The conformational changes of a 22 base-pair double-stranded DNA, anchored via one end to a quartz substrate, have been characterized using a single-pair fluorescence resonance energy transfer technique. Base-pair mismatch, a major form of DNA damage, has been found to decrease the energy transfer between a fluorescence donor and an acceptor attached to the two ends of DNA molecules with 3 and 7 mismatches, by 4.4% and 10.4%, respectively, as compared to that for perfectly matched DNA. This result suggests that the disruption of the hydrogen bonds in damaged DNA leads to increased contour length and improved flexibility.
AB - The conformational changes of a 22 base-pair double-stranded DNA, anchored via one end to a quartz substrate, have been characterized using a single-pair fluorescence resonance energy transfer technique. Base-pair mismatch, a major form of DNA damage, has been found to decrease the energy transfer between a fluorescence donor and an acceptor attached to the two ends of DNA molecules with 3 and 7 mismatches, by 4.4% and 10.4%, respectively, as compared to that for perfectly matched DNA. This result suggests that the disruption of the hydrogen bonds in damaged DNA leads to increased contour length and improved flexibility.
UR - http://www.scopus.com/inward/record.url?scp=24144449890&partnerID=8YFLogxK
U2 - 10.1063/1.1997279
DO - 10.1063/1.1997279
M3 - Article
AN - SCOPUS:24144449890
VL - 87
JO - Applied Physics Letters
JF - Applied Physics Letters
SN - 0003-6951
IS - 3
M1 - 033901
ER -