Single grating x-ray imaging for dynamic biological systems

Kaye S. Morgan, David M. Paganin, David Webb Parsons, Martin Donnelley, Naoto Yagi, Kentaro Uesugi, Yoshio Suzuki, Akihisa Takeuchi, Karen K W Siu

Research output: Chapter in Book/Report/Conference proceedingConference PaperResearch

3 Citations (Scopus)


Biomedical studies are already benefiting from the excellent contrast offered by phase contrast x-ray imaging, but live imaging work presents several challenges. Living samples make it particularly difficult to achieve high resolution, sensitive phase contrast images, as exposures must be short and cannot be repeated. We therefore present a single-exposure, high-flux method of differential phase contrast imaging [1, 2, 3] in the context of imaging live airways for Cystic Fibrosis (CF) treatment assessment [4]. The CF study seeks to non-invasively observe the liquid lining the airways, which should increase in depth in response to effective treatments. Both high spatial resolution and sensitivity are required in order to track micron size changes in a liquid that is not easily differentiated from the tissue on which it lies. Our imaging method achieves these goals by using a single attenuation grating or grid as a reference pattern, and analyzing how the sample deforms the pattern to quantitatively retrieve the phase depth of the sample. The deformations are mapped at each pixel in the image using local cross-correlations comparing each 'sample and pattern' image with a reference 'pattern only' image taken before the sample is introduced. This produces a differential phase image, which may be integrated to give the sample phase depth.

Original languageEnglish
Title of host publicationInternational Workshop on X-Ray and Neutron Phase Imaging with Gratings
EditorsAtsushi Momose, Wataru Yashiro
PublisherAmerican Institute of Physics
Number of pages6
ISBN (Print)9780735410725
Publication statusPublished - 2012
EventInternational Workshop on X-ray and Neutron Phase Imaging with Gratings (XNPIG) 2012 - National Museum of Emerging Science and Innovation (Miraikan), Tokyo, Japan
Duration: 5 Mar 20127 Mar 2012

Publication series

NameAIP Conference Proceedings
PublisherAmerican Institute of Physics
ISSN (Print)0094-243X


WorkshopInternational Workshop on X-ray and Neutron Phase Imaging with Gratings (XNPIG) 2012
Abbreviated titleXNPIG 2012
OtherMonash resarcher has confirmed that their conference paper was not peer reviewed
Internet address


  • Airways
  • Biomedical imaging
  • Cystic Fibrosis
  • Phase contrast x-ray imaging
  • Synchrotron imaging

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