Single channel Cl- and K+ currents from cells of uterus not treated with enzymes

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Single channel currents were recorded from uterine smooth muscle cells of pregnant guinea-pigs. The cells were within small bundles of smooth muscle of low input resistance. Giga-ohm seals were formed between the patch clamp electrodes and the membrane of smooth muscle cells that had not been treated with enzymes. In cell-attached mode, outward current steps were observed that had a conductance of 130-170 pS, a reversal potential close to the potassium reversal potential, and the probability of a channel being in the open state increased e-fold per 8-11 mV depolarization. In the cell-free, inside-out patch mode, currents were recorded that had a large conductance, 420 pS, and smaller conductance levels. When the chloride concentration was changed the reversal potential shifted in a manner consistent with the Nernst equation indicating that the channels were permeable to chloride ions.

Original languageEnglish
Pages (from-to)560-562
Number of pages3
JournalPflügers Archiv European Journal of Physiology
Issue number4-5
Publication statusPublished - 1 Nov 1987


  • channels in myometrium
  • chloride channels
  • patch clamp
  • potassium channels

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