Single-chain Fv antibodies for targeting neurodegenerative diseases

Kin Yen Chia, Khuen Yen Ng, Rhun Yian Koh, Soi Moi Chye

Research output: Contribution to journalReview ArticleResearchpeer-review

12 Citations (Scopus)

Abstract

Background & Objective: Protein misfolding and aggregation have been considered the common pathological hallmarks for a number of neurodegenerative diseases, including Alzheimer’s disease (AD), Parkinson’s disease (PD) and Huntington’s disease (HD). These abnormal proteins aggregates damage mitochondria and induce oxidative stress, resulting in neuronal cell death. Prolonged neuronal damage activates microglia and astrocytes, development of inflammation reaction and further promotes neurodegeneration. Thus, elimination of abnormal protein aggregates without eliciting any adverse effects are the main treatment strategies. To overcome this, recent studies have deployed single-chain fragment variable antibodies (scFvs) to target the pathological protein aggregates, such as amyloid-beta (Aβ) peptides, α-synuclein (α-syn) and Huntingtin (Htt). To date scFv has been effective at inhibiting abnormal protein aggregates formation in both in vitro and in vivo model system of AD, PD and HD. Conclusion: Currently active research is still ongoing to improve the scFv gene delivery technology, to further enhance brain penetration, intracellular stability, solubility and efficacy of scFv intrabody.

Original languageEnglish
Pages (from-to)671-679
Number of pages9
JournalCNS & Neurological Disorders - Drug Targets
Volume17
Issue number9
DOIs
Publication statusPublished - 2018

Keywords

  • Alzheimer’s disease
  • Inflammation reaction
  • Neurodegenerative disease
  • Oxidative stress
  • Pathological
  • Single-chain Fv antibodies

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