Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 2855-2863 |
| Number of pages | 9 |
| Journal | Molecular Pharmaceutics |
| Volume | 11 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - 2014 |
| Externally published | Yes |
Cite this
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Single-Chain antibody conjugated to a cage amine chelator and labeled with positron-emitting copper-64 for diagnostic imaging of activated platelets. / Alt, Karen Maria; Paterson, Brett M; Ardipradja, Katie; Schieber, Christine; Buncic, Gojko; Lim, Bock; Poniger, Stan; Jakoby, Bjoern; Wang, Xiaowei; O'Keefe, Graeme J; Tochon-Danguy, Henri; Scott, Andrew Mark; Ackermann, Uwe; Peter, Karlheinz; Donnelly, Paul Stephen; Hagemeyer, Christoph Eugen.
In: Molecular Pharmaceutics, Vol. 11, No. 8, 2014, p. 2855-2863.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Single-Chain antibody conjugated to a cage amine chelator and labeled with positron-emitting copper-64 for diagnostic imaging of activated platelets
AU - Alt, Karen Maria
AU - Paterson, Brett M
AU - Ardipradja, Katie
AU - Schieber, Christine
AU - Buncic, Gojko
AU - Lim, Bock
AU - Poniger, Stan
AU - Jakoby, Bjoern
AU - Wang, Xiaowei
AU - O'Keefe, Graeme J
AU - Tochon-Danguy, Henri
AU - Scott, Andrew Mark
AU - Ackermann, Uwe
AU - Peter, Karlheinz
AU - Donnelly, Paul Stephen
AU - Hagemeyer, Christoph Eugen
PY - 2014
Y1 - 2014
N2 - Imaging of activated platelets using an activation specific anti-GPIIb/IIIa integrin single-chain antibody (scFvanti-LIBS) conjugated to a positron emitting copper-64 complex of a cage amine sarcophagine chelator (MeCOSar) is reported. This tracer was compared in vitro to a 64CuII complex of the scFv conjugated to another commonly used macrocycle, DOTA. The scFvanti-LIBS-MeCOSar conjugate was radiolabeled with 64CuII rapidly under mild conditions and with higher specific activity than scFvanti-LIBS-DOTA. The utility of scFvanti-LIBS-MeCOSar as a diagnostic agent was assessed in vivo in a mouse model of acute thrombosis. The uptake of scFvanti-LIBS- 64CuMeCOSar in the injured vessel was significantly higher than the noninjured vessel. Positron emission tomography (PET) was used to show accumulation of scFvanti-LIBS-64CuMeCOSar with high and specific uptake in the injured vessel. ScFvanti-LIBS- 64CuMeCOSar is an excellent tool for highly sensitive in vivo detection of activated platelets in PET and has the potential to be used for early diagnosis of acute thrombotic events.
AB - Imaging of activated platelets using an activation specific anti-GPIIb/IIIa integrin single-chain antibody (scFvanti-LIBS) conjugated to a positron emitting copper-64 complex of a cage amine sarcophagine chelator (MeCOSar) is reported. This tracer was compared in vitro to a 64CuII complex of the scFv conjugated to another commonly used macrocycle, DOTA. The scFvanti-LIBS-MeCOSar conjugate was radiolabeled with 64CuII rapidly under mild conditions and with higher specific activity than scFvanti-LIBS-DOTA. The utility of scFvanti-LIBS-MeCOSar as a diagnostic agent was assessed in vivo in a mouse model of acute thrombosis. The uptake of scFvanti-LIBS- 64CuMeCOSar in the injured vessel was significantly higher than the noninjured vessel. Positron emission tomography (PET) was used to show accumulation of scFvanti-LIBS-64CuMeCOSar with high and specific uptake in the injured vessel. ScFvanti-LIBS- 64CuMeCOSar is an excellent tool for highly sensitive in vivo detection of activated platelets in PET and has the potential to be used for early diagnosis of acute thrombotic events.
UR - http://pubs.acs.org/doi/abs/10.1021/mp500209a
U2 - 10.1021/mp500209a
DO - 10.1021/mp500209a
M3 - Article
VL - 11
SP - 2855
EP - 2863
JO - Molecular Pharmaceutics
JF - Molecular Pharmaceutics
SN - 1543-8384
IS - 8
ER -