TY - JOUR
T1 - Single-atom nanozymes linked immunosorbent assay for sensitive detection of aβ 1-40
T2 - A biomarker of alzheimer's disease
AU - Lyu, Zhaoyuan
AU - Ding, Shichao
AU - Zhang, Nan
AU - Zhou, Yang
AU - Cheng, Nan
AU - Wang, Maoyu
AU - Xu, Mingjie
AU - Feng, Zhenxing
AU - Niu, Xiangheng
AU - Cheng, Yuan
AU - Zhang, Chao
AU - Du, Dan
AU - Lin, Yuehe
N1 - Publisher Copyright:
© 2020 Zhaoyuan Lyu et al.
PY - 2020/10/19
Y1 - 2020/10/19
N2 - Single-atom nanozymes (SANs) possess unique features of maximum atomic utilization and present highly assembled enzyme-like structure and remarkable enzyme-like activity. By introducing SANs into immunoassay, limitations of ELISA such as low stability of horseradish peroxidase (HRP) can be well addressed, thereby improving the performance of the immunoassays. In this work, we have developed novel Fe-N-C single-atom nanozymes (Fe-Nx SANs) derived from Fe-doped polypyrrole (PPy) nanotube and substituted the enzymes in ELISA kit for enhancing the detection sensitivity of amyloid beta 1-40. Results indicate that the Fe- Nx SANs contain high density of single-atom active sites and comparable enzyme-like properties as HRP, owing to the maximized utilization of Fe atoms and their abundant active sites, which could mimic natural metalloproteases structures. Further designed SAN-linked immunosorbent assay (SAN-LISA) demonstrates the ultralow limit of detection (LOD) of 0.88 pg/mL, much more sensitive than that of commercial ELISA (9.98 pg/mL). The results confirm that the Fe-Nx SANs can serve as a satisfactory replacement of enzyme labels, which show great potential as an ultrasensitive colorimetric immunoassay.
AB - Single-atom nanozymes (SANs) possess unique features of maximum atomic utilization and present highly assembled enzyme-like structure and remarkable enzyme-like activity. By introducing SANs into immunoassay, limitations of ELISA such as low stability of horseradish peroxidase (HRP) can be well addressed, thereby improving the performance of the immunoassays. In this work, we have developed novel Fe-N-C single-atom nanozymes (Fe-Nx SANs) derived from Fe-doped polypyrrole (PPy) nanotube and substituted the enzymes in ELISA kit for enhancing the detection sensitivity of amyloid beta 1-40. Results indicate that the Fe- Nx SANs contain high density of single-atom active sites and comparable enzyme-like properties as HRP, owing to the maximized utilization of Fe atoms and their abundant active sites, which could mimic natural metalloproteases structures. Further designed SAN-linked immunosorbent assay (SAN-LISA) demonstrates the ultralow limit of detection (LOD) of 0.88 pg/mL, much more sensitive than that of commercial ELISA (9.98 pg/mL). The results confirm that the Fe-Nx SANs can serve as a satisfactory replacement of enzyme labels, which show great potential as an ultrasensitive colorimetric immunoassay.
UR - http://www.scopus.com/inward/record.url?scp=85096034207&partnerID=8YFLogxK
U2 - 10.34133/2020/4724505
DO - 10.34133/2020/4724505
M3 - Article
AN - SCOPUS:85096034207
SN - 2096-5168
VL - 2020
JO - Research
JF - Research
M1 - 4724505
ER -