Abstract
A simple and rapid gas chromatographic method for the quantitation of chlormethiazole and two of its pharmacologically active metabolites, 5-acetyl-4-methylthiazole and 5-(1-hydroxyethyl)-4-methylthiazole, in plasma and blood is described. The total analysis time is less than 20 min for a single sample. The method requires 50-500 μl of plasma or 1 ml of blood. The compounds are detected with a nitrogen-phosphorus detector. An internal standard technique is used for the quantitation. Calibration data are linear over the range 32-2376 ng of chlormethiazole and a similar range of the metabolites in plasma. The method may be used for pharmacokinetic studies.
| Original language | English |
|---|---|
| Pages (from-to) | 55-62 |
| Number of pages | 8 |
| Journal | Journal of Chromatography B: Biomedical Sciences and Applications |
| Volume | 182 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 11 Apr 1980 |