Simultaneous quantitation of chlormethiazole and two of its metabolites in blood and plasma by gas-liquid chromatography

S. Edmund Tsuei, Jack Thomas, Roger L. Nation

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A simple and rapid gas chromatographic method for the quantitation of chlormethiazole and two of its pharmacologically active metabolites, 5-acetyl-4-methylthiazole and 5-(1-hydroxyethyl)-4-methylthiazole, in plasma and blood is described. The total analysis time is less than 20 min for a single sample. The method requires 50-500 μl of plasma or 1 ml of blood. The compounds are detected with a nitrogen-phosphorus detector. An internal standard technique is used for the quantitation. Calibration data are linear over the range 32-2376 ng of chlormethiazole and a similar range of the metabolites in plasma. The method may be used for pharmacokinetic studies.

Original languageEnglish
Pages (from-to)55-62
Number of pages8
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Issue number1
Publication statusPublished - 11 Apr 1980

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