Simultaneous quantitation of chlormethiazole and two of its metabolites in blood and plasma by gas-liquid chromatography

S. Edmund Tsuei, Jack Thomas, Roger L. Nation

Research output: Contribution to journalArticleResearchpeer-review

Abstract

A simple and rapid gas chromatographic method for the quantitation of chlormethiazole and two of its pharmacologically active metabolites, 5-acetyl-4-methylthiazole and 5-(1-hydroxyethyl)-4-methylthiazole, in plasma and blood is described. The total analysis time is less than 20 min for a single sample. The method requires 50-500 μl of plasma or 1 ml of blood. The compounds are detected with a nitrogen-phosphorus detector. An internal standard technique is used for the quantitation. Calibration data are linear over the range 32-2376 ng of chlormethiazole and a similar range of the metabolites in plasma. The method may be used for pharmacokinetic studies.

Original languageEnglish
Pages (from-to)55-62
Number of pages8
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume182
Issue number1
DOIs
Publication statusPublished - 11 Apr 1980

Cite this

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abstract = "A simple and rapid gas chromatographic method for the quantitation of chlormethiazole and two of its pharmacologically active metabolites, 5-acetyl-4-methylthiazole and 5-(1-hydroxyethyl)-4-methylthiazole, in plasma and blood is described. The total analysis time is less than 20 min for a single sample. The method requires 50-500 μl of plasma or 1 ml of blood. The compounds are detected with a nitrogen-phosphorus detector. An internal standard technique is used for the quantitation. Calibration data are linear over the range 32-2376 ng of chlormethiazole and a similar range of the metabolites in plasma. The method may be used for pharmacokinetic studies.",
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Simultaneous quantitation of chlormethiazole and two of its metabolites in blood and plasma by gas-liquid chromatography. / Tsuei, S. Edmund; Thomas, Jack; Nation, Roger L.

In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 182, No. 1, 11.04.1980, p. 55-62.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Simultaneous quantitation of chlormethiazole and two of its metabolites in blood and plasma by gas-liquid chromatography

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AB - A simple and rapid gas chromatographic method for the quantitation of chlormethiazole and two of its pharmacologically active metabolites, 5-acetyl-4-methylthiazole and 5-(1-hydroxyethyl)-4-methylthiazole, in plasma and blood is described. The total analysis time is less than 20 min for a single sample. The method requires 50-500 μl of plasma or 1 ml of blood. The compounds are detected with a nitrogen-phosphorus detector. An internal standard technique is used for the quantitation. Calibration data are linear over the range 32-2376 ng of chlormethiazole and a similar range of the metabolites in plasma. The method may be used for pharmacokinetic studies.

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