A simple and rapid gas chromatographic method for the quantitation of chlormethiazole and two of its pharmacologically active metabolites, 5-acetyl-4-methylthiazole and 5-(1-hydroxyethyl)-4-methylthiazole, in plasma and blood is described. The total analysis time is less than 20 min for a single sample. The method requires 50-500 μl of plasma or 1 ml of blood. The compounds are detected with a nitrogen-phosphorus detector. An internal standard technique is used for the quantitation. Calibration data are linear over the range 32-2376 ng of chlormethiazole and a similar range of the metabolites in plasma. The method may be used for pharmacokinetic studies.
|Number of pages
|Journal of Chromatography B: Biomedical Sciences and Applications
|Published - 11 Apr 1980